L27

L27 or L-27 may refer to:
 * L27 domain, a protein domain
 * 60S ribosomal protein L27
 * Buick L27 engine, a V6 automobile engine
 * Cessna L-27, a light utility aircraft of the U.S. Air Force
 * HMS L27, a submarine of the Royal Navy
 * HMS Hastings (L27), a sloop of the Royal Navy
 * Klemm L 27, a German training aircraft
 * Lectionary 27, a medieval Greek manuscript