List of Billboard Tropical Airplay number ones of 2000

Tropical Airplay (formerly designated as Hot Tropical/Salsa Tracks) is a chart published by Billboard magazine that ranks the top-performing songs (regardless of genre or language) on tropical radio stations in the United States, based on weekly airplay data compiled by Nielsen's Broadcast Data Systems. It is a subchart of Hot Latin Songs (formerly known as Hot Latin Tracks), which lists the best-performing Spanish-language songs in the country. In 2000, 15 songs topped the chart, across 52 issues of the magazine.

The first number one of the year was "Pero Dile" by Víctor Manuelle, which had been in the top spot since the issue dated November 13, 1999. It was succeeded by Carlos Vives's first number one on the chart with "Fruta Fresca". The two songs alternated on the top stop until the week of January 29, 2000, when Grupo Manía reached number-one with "Bajo la Lluvía". "A Puro Dolor" had the longest-running number of the year with ten weeks and was the best-performing tropical song of the year as well. Son by Four also had the final one of the year with "Cuando Seas Mía", the Spanish version of "Miss Me So Bad".

Four songs that reached number one in 2000 are Spanish-language adaptations of English-language songs, including "Cuando Seas Mía" ("Miss Me So Bad") by Son by Four, "Muy Dentro de Mi" ("You Sang to Me") by Marc Anthony, "Ven Conmigo (Solamente Tú)" ("Come On Over Baby (All I Want Is You)") by Christina Aguilera, and the Spanish version of "She Bangs" by Ricky Martin. Thalía and Luis Fonsi achieved their first number ones with "Entre el Mar y una Estrella" and "Imagíname Sin Ti", respectively. Due to the fact that tropical radio stations do not generally play pop ballads, Thalía's record label, EMI Latin, was suspected of performing payola and was removed from the chart the following week. Former La Makina lead vocalist Anthony achieved his only chart-topper with "Yo Te Confieso".