List of Minnesota Twins minor league affiliates

The Minnesota Twins farm system consists of six Minor League Baseball affiliates across the United States and in the Dominican Republic. Four teams are independently owned, while two—the Florida Complex League Twins and Dominican Summer League Twins—are owned by the major league club.

The Twins have been affiliated with the Single-A Fort Myers Mighty Mussels of the Florida State League since 1993, making it the longest-running active affiliation in the organization among teams not owned by the Twins. The longest affiliation in franchise history was with the Elizabethton Twins, who were the team's Rookie affiliate in the Appalachian League for 47 seasons from 1974 to 2020. Their newest affiliates are the Triple-A St. Paul Saints of the Pacific Coast League and the Double-A Wichita Wind Surge of the Texas League, which became Twins affiliates in 2021.

Geographically, Minnesota's closest domestic affiliate is the St. Paul Saints, which are located approximately 10 mi away. Their furthest domestic affiliates are the Fort Myers Mighty Mussels and Florida Complex League Twins of the Rookie Florida Complex League, which play at different ballparks at the same facility some 1423 mi away.

Current affiliates
The Minnesota Twins farm system consists of six minor league affiliates.

1932–1962
Minor League Baseball operated with five classes (Double-A, Class A, Class B, Class C, and Class D) from 1932 to 1935. Class A1, between Double-A and Class A, was added in 1936. The minors continued to operate with these six levels through 1945. Triple-A was established as the highest classification in 1946, and Class A1 became Double-A, with Class A through D remaining. These six levels continued through 1962. The Pacific Coast League (PCL) was reclassified from Triple-A to Open in 1952 due to the possibility of becoming a third major league. This arrangement ended following the 1957 season when the relocation of the National League's Dodgers and Giants to the West Coast ended any chance of the PCL being promoted.

1963–1989
Prior to the 1963 season, Major League Baseball (MLB) initiated a reorganization of Minor League Baseball that resulted in a reduction from six classes to four (Triple-A, Double-A, Class A, and Rookie) in response to the general decline of the minors throughout the 1950s and early-1960s when leagues and teams folded due to shrinking attendance caused by baseball fans' preference for staying at home to watch MLB games on television. The only change made within the next 27 years was Class A being subdivided for the first time to form Class A Short Season in 1966.

1990–2020
Minor League Baseball operated with six classes from 1990 to 2020. In 1990, the Class A level was subdivided for a second time with the creation of Class A-Advanced. The Rookie level consisted of domestic and foreign circuits. The Twins did not field a Class A Short Season team during this period.

2021–present
The current structure of Minor League Baseball is the result of an overall contraction of the system beginning with the 2021 season. Class A was reduced to two levels: High-A and Low-A. Low-A was reclassified as Single-A in 2022.