List of U.S. Class II railroads

In the United States, a Class II railroad, sometimes referred to as a regional railroad, is a railroad company that is not Class I, but still has a substantial amount of traffic or trackage (and is thus not a short line). The Association of American Railroads (AAR) has defined the lower bound as 350 mi of track or $40 million in annual operating revenue. (The Class I threshold is $250 million, adjusted for inflation since 1991. )., a Class II railroad in the United States has an operating revenue greater than $39.2 million but less than $489.9 million.

Former Class II railroads

 * Aberdeen and Rockfish Railroad
 * Alabama Great Southern Railroad (AGS), owned by Norfolk Southern Railway
 * Atlanta and West Point Railroad (AWP), owned by the Atlantic Coast Line Railroad
 * Central Oregon and Pacific Railroad (CORP)
 * New York, Ontario & Western (NYOW)
 * Lehigh & Hudson River Railway (LHR)
 * Lehigh & New England (LNE)
 * Bessemer and Lake Erie Railroad (BLE), owned by Canadian National Railway
 * Bullfrog Goldfield Railroad, incorporated by interests of the Tonopah and Goldfield Railroad , later merged with the Tonopah and Tidewater and the Las Vegas and Tonopah.
 * Central of Georgia Railroad (CG), owned by Norfolk Southern Railway
 * Central Maine and Quebec Railway (CMQ), owned by Canadian Pacific Railway
 * Bangor and Aroostook Railroad (BAR)
 * Chicago Central and Pacific Railroad
 * Chicago, Missouri and Western Railway
 * Carolina and Northwestern Railway
 * Central Vermont Railway (CV), owned by Canadian National Railway (CN)
 * Dakota, Minnesota and Eastern Railroad (DME), owned by Canadian Pacific Railway
 * Duluth, Missabe and Iron Range Railway (DMIR), owned by Canadian National Railway
 * Detroit and Toledo Shore Line Railroad (DTS)
 * Elgin, Joliet and Eastern Railway (EJE), owned by Canadian National Railway
 * Fox Valley and Western Ltd. (FVW), owned by Canadian National Railway
 * Gateway Western Railway (GWWR), owned by Kansas City Southern Railway
 * Georgia Southern and Florida Railway (GSF), owned by Norfolk Southern Railway
 * Georgia Railroad and Banking Company (GA) owned by Seaboard Coast Line Railroad (SCL)
 * Great Lakes Central Railroad (GLC)
 * Gulf and Mississippi Railroad (GMSR)
 * I&M Rail Link (IMRL)
 * Iowa, Chicago and Eastern Railroad (ICE), owned by Canadian Pacific Railway
 * Las Vegas and Tonopah Railroad
 * Magma Arizona Railroad
 * Minarets and Western Railway
 * Monongahela Railway
 * Montana Rail Link (MRL), until its reintegration into BNSF on January 1st, 2024
 * Montreal, Maine and Atlantic Railway (MMA)
 * MidSouth Rail Corporation (MSRC)
 * Minneapolis, Northfield and Southern Railway
 * Oklahoma, Kansas and Texas Railroad
 * Pan Am Railways (PAR), acquired by CSX Transportation
 * Richmond, Fredericksburg and Potomac Railroad (RFP)
 * Spokane International Railroad
 * Tonopah and Goldfield Railroad
 * Tonopah and Tidewater Railroad
 * Texas Mexican Railway (TM), owned by Kansas City Southern Railway
 * Wisconsin Central Ltd. (WC), owned by Canadian National Railway
 * Western Railway of Alabama (WofA) owned by the Atlantic Coast Line Railroad