List of highways numbered 301

Route 301 or Highway 301 could refer to:

Canada

 * Manitoba secondary 301.svg Manitoba Provincial Road 301
 * NL Route 301.svg Newfoundland and Labrador Route 301
 * Nova Scotia Route 301.svg Nova Scotia Route 301
 * PEI_Highway_301.svg Prince Edward Island Route 301
 * Qc301.svg Quebec Route 301
 * Saskatchewan Highway 301 (jct).svg Saskatchewan Highway 301

China

 * Kokudou 301(China).svg China National Highway 301

Costa Rica

 * CR RNT 301.svg National Route 301

Japan

 * Japanese National Route Sign 0301.svg Japan National Route 301

Korea, South

 * Korea Expressway No.301.svg Sangju–Yeongcheon Expressway

Philippines

 * N301 (Philippines).svg N301 highway (Philippines)

United States

 * US 301 Virginia 1926.svg U.S. Route 301 (former)
 * US 301.svg U.S. Route 301
 * Arkansas 301.svg Arkansas Highway 301
 * Georgia 301.svg Georgia State Route 301
 * Indiana 301.svg Indiana State Road 301
 * Iowa 301.svg Iowa Highway 301 (former)
 * Elongated circle 301.svg Kentucky Route 301
 * Louisiana 301.svg Louisiana Highway 301
 * MD Route 301.svg Maryland Route 301
 * MN-301.svg Minnesota State Highway 301
 * Ellipse sign 301.svg Mississippi Highway 301
 * New York:
 * NY-301.svg New York State Route 301
 * Albany County 301 wide.svg County Route 301 (Albany County, New York)
 * Erie County 301 NY.svg County Route 301 (Erie County, New York)
 * County Route 301 (Westchester County, New York)
 * NC 301.svg North Carolina Highway 301 (former)
 * OH-301.svg Ohio State Route 301
 * PA-301.svg Pennsylvania Route 301 (former)
 * Secondary Tennessee 301.svg Tennessee State Route 301
 * Texas:
 * Texas 301.svg Texas State Highway 301 (former)
 * Texas Loop 301.svg Texas State Highway Loop 301
 * Texas FM 301.svg Farm to Market Road 301
 * Utah SR 301.svg Utah State Route 301
 * Virginia 301.svg Virginia State Route 301

Other areas:
 * Ellipse sign 301.svg Puerto Rico Highway 301
 * Ellipse sign 301.svg U.S. Virgin Islands Highway 301

Film

 * Highway 301 (film), a 1950 film noir by Andrew L. Stone