List of highways numbered 331

Route 331, or Highway 331, may refer to:

Australia
- Myrtle Street

Canada

 * Manitoba secondary 331.svg Manitoba Provincial Road 331
 * NL Route 331.svg Newfoundland and Labrador Route 331
 * Nova Scotia Route 331.svg Nova Scotia Route 331
 * PEI_Highway_331.svg Prince Edward Island Route 331

Costa Rica

 * CR RNT 331.svg National Route 331

Hungary

 * 331 (Hu) Otszogletu zold tabla.svg Main road 331 (Hungary)

India

 * NH331-IN.svg National Highway 331 (India)

Japan

 * Japanese National Route Sign 0331.svg Japan National Route 331

United States

 * US 331 (1948).svg U.S. Route 331 (former)
 * US 331.svg U.S. Route 331
 * Arkansas 331.svg Arkansas Highway 331
 * Florida:
 * Florida 331.svg Florida State Road 331
 * Levy County 331A.svg County Road 331A (Levy County, Florida)
 * Georgia 331.svg Georgia State Route 331
 * Indiana 331.svg Indiana State Road 331
 * Elongated circle 331.svg Kentucky Route 331
 * Louisiana 331.svg Louisiana Highway 331
 * MD Route 331.svg Maryland Route 331
 * M-331.svg M-331 (Michigan highway)
 * Ellipse sign 331.svg Mississippi Highway 331 (unsigned)
 * MT-sec-331.svg Montana Secondary Highway 331
 * NY-331.svg New York State Route 331
 * OH-331.svg Ohio State Route 331
 * OR 331.svg Oregon Route 331
 * PA-331.svg Pennsylvania Route 331
 * Ellipse sign 331.svg Puerto Rico Highway 331
 * Secondary Tennessee 331.svg Tennessee State Route 331
 * Texas:
 * Texas 331.svg Texas State Highway 331 (former)
 * Texas Spur 331.svg Texas State Highway Spur 331
 * Texas FM 331.svg Farm to Market Road 331
 * Virginia 331.png Virginia State Route 331
 * WV-331.svg West Virginia Route 331
 * WY-331.svg Wyoming Highway 331