List of museums in Ontario

This list of museums in Ontario, Canada contains museums which are defined for this context as institutions (including nonprofit organizations, government entities, and private businesses) that collect and care for objects of cultural, artistic, scientific, or historical interest and make their collections or related exhibits available for public viewing. Also included are non-profit art galleries and university art galleries. Museums that exist only in cyberspace (i.e., virtual museums) are not included.

See also List of museums in Ottawa for museums in the city of Ottawa. See also List of museums in Toronto for museums in the city of Toronto.

Defunct museums

 * Canadian Ski Museum, moved from Ottawa to Mont-Tremblant, Quebec in 2013
 * Chesley Heritage & Woodworking Museum, Chesley, closed in 2013
 * Clark House Museum, Powassan, closed 2012
 * Criminals Hall of Fame, Niagara Falls, closed 2014
 * Dairy Museum, Aylmer
 * Edison Museum, Vienna, closed 2016
 * Elliott Avedon Museum and Archive of Games, closed in 2009, collections now at the Canadian Museum of Civilization
 * First Hussars Museum, London
 * Guy Lombardo Museum, London, closed in 2007
 * Innisville Museum, Innisville, closed 2007
 * Jolley's Riding Toy Museum, Meaford, closed 2016
 * Kingston Archaeological Centre, Kingston
 * Mahoney Dolls House Gallery, Fort Erie, closed 2010
 * Memory Junction Railway Museum, Brighton, closed 2018
 * MS Norgoma, Sault Ste. Marie, closed 2019
 * The Niagara Falls Museum, Niagara Falls
 * Personal Computer Museum, Brantford, closed 2018
 * Revolution Frères Museum, Moosonee
 * Riverview Miniature Village, Peterborough, closed 2014
 * Scugog Shores Heritage Centre and Archives, Port Perry, closed 2019
 * Seagram Museum, Waterloo
 * Shania Twain Centre, Timmins, closed in 2013
 * Timmins Underground Gold Mine Tour, Timmins, closed in 2013
 * Woodstock Peace Lighthouse, Woodstock, closed circa 2016