List of teams and cyclists in the 2010 Giro d'Italia

The 2010 Giro d'Italia began on May 8 in Amsterdam in the Netherlands, and ended on May 30 in Verona. Twenty-two professional cycling teams entered the race, who each entered a squad of nine riders. This group includes 15 UCI ProTour teams and seven UCI Professional Continental teams. Sixteen teams were guaranteed entry by a September 2008 contract between the UCI and the organizers of the season's three Grand Tours. Two from this group – and  – declined to participate in the race, freeing up spots for two more teams. Two new teams joined the ProTour in 2010, but only one,, participated in the Giro, as opted instead to send their best riders to the 2010 Tour of California, which was partly concurrent to the Giro.

Of the seven Professional Continental teams in the race, two, and, were covered by the September 2008 contract, as they were members of the ProTour at that time. The other five Professional Continental teams had to be selected by race organizers. There was minor controversy in that although the Giro begins in the Netherlands, the only Dutch-registered team in the race was, which was guaranteed entry. and both sought places in the race, but did not receive them.