Lotis

Lotis can refer to:
 * Lotis (mythology), a nymph in Greek mythology
 * Lotis (beetle), a genus of beetles
 * Large Optical Test and Integration Site, at the Lockheed Martin Space Systems Company in Sunnyvale, CA
 * Livermore Optical Transient Imaging System, an automated telescope for follow-up of gamma ray bursts
 * LOTIS committee, the Liberalisation of Trade in Services Committee, a working group within TheCityUK, United Kingdom
 * 429 Lotis, a main belt asteroid

People with the name

 * Lotis Key, former Filipino-American professional film and theater actress
 * Dennis Lotis (1925–2023), South African-born British singer, actor, and entertainer