Middlebrook 7H10 Agar

Middlebrook 7H10 Agar is a solid growth medium specially used for culture of Mycobacterium, notably Mycobacterium tuberculosis. It has been reported that the 7H10 medium tends to grow fewer contaminants than the egg-based media commonly used for the cultivation of mycobacteria.

Composition
Ingredients (g/L)
 * Ammonium sulfate, 0.50
 * Monopotassium phosphate, 1.50
 * Disodium phosphate, 1.50
 * Sodium citrate, 0.40
 * Magnesium sulfate, 0.025
 * Zinc sulfate, 0.001
 * Copper sulfate, 0.001
 * L-Glutamic acid, 0.50
 * Ferric ammonium citrate, 0.04
 * Pyridoxine hydrochloride, 0.001
 * Biotin, 0.0005
 * Malachite green, 0.00025
 * Agar, 15.00
 * Calcium chloride, 0.0005

Cultures should be read within 5–7 days after inoculation and once a week thereafter for up to 8 weeks.