Multisite-specific tRNA:(cytosine-C5)-methyltransferase

Multisite-specific tRNA:(cytosine-C5)-methyltransferase (, multisite-specific tRNA:m5C-methyltransferase, TRM4 (gene)) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (cytosine-C5)-methyltransferase. This enzyme catalyses the following chemical reaction


 * (1) S-adenosyl-L-methionine + cytosine34 in tRNA precursor $$\rightleftharpoons$$ S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor
 * (2) S-adenosyl-L-methionine + cytosine40 in tRNA precursor $$\rightleftharpoons$$ S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA precursor
 * (3) S-adenosyl-L-methionine + cytosine48 in tRNA $$\rightleftharpoons$$ S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
 * (4) S-adenosyl-L-methionine + cytosine49 in tRNA $$\rightleftharpoons$$ S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA

The enzyme from Saccharomyces cerevisiae is responsible for complete 5-methylcytosine methylations of yeast tRNA.