Phenylalanine N-monooxygenase

Phenylalanine N-monooxygenase (, phenylalanine N-hydroxylase, CYP79A2) is an enzyme with systematic name L-phenylalanine,NADPH:oxygen oxidoreductase (N-hydroxylating). This enzyme catalyses the following chemical reaction


 * L-phenylalanine + 2 O2 + 2 NADPH + 2 H+ $$\rightleftharpoons$$ (E)-phenylacetaldoxime + 2 NADP+ + CO2 + 3 H2O (overall reaction)
 * (1a) L-phenylalanine + O2 + NADPH + H+ $$\rightleftharpoons$$ N-hydroxy-L-phenylalanine + NADP+ + H2O:
 * (1b) N-hydroxy-L-phenylalanine + O2 + NADPH + H+ $$\rightleftharpoons$$ N,N-dihydroxy-L-phenylalanine + NADP+ + H2O
 * (1c) N,N-dihydroxy-L-phenylalanine $$\rightleftharpoons$$ (E)-phenylacetaldoxime + CO2 + H2O

Phenylalanine N-monooxygenase is a heme-thiolate protein (P-450). It is part of the pathway in plants which converts phenylalanine to the glucosinolate, glucotropaeolin, which contributes to the characteristic flavor of brassicas.