Pyrylium-1

Pyrylium-1 (Py-1) is a fluorogenic salt of a pyrylium derivative and tetrafluoroborate. It is an amine-labeling dye that is not fluorescent itself, but reacts with primary amines to form fluorescent products. It is within the "chameleon labels" class, so named due to their clear color-changing properties upon conjugation. Py-1 was first reported in 2004. It has been used for the detection of amines and peptides, largely in CE-SDS, where it is recognized to reach a high sensitivity via laser-induced fluorescence. Once bound to protein the excitation wavelength is 503 nm (green) and the emission wavelength is 603 nm (orange). Similar to FQ, these fluorescence wavelengths makes Py-1 suitable for excitation with a 488 nm argon-ion laser.

Reaction


Post conjugation, the Py-1 adduct (an addition of C21H21N) adds about 287.1674 Da to the target molecule.