RLO

RLO may refer to:
 * Het Rijnlands Lyceum Oegstgeest, a secondary school in the town of Oegstgeest in South Holland
 * Right-to-left override, the Unicode control character
 * Valle del Conlara Airport, the IATA code RLO
 * Radio Limerick One, a licensed radio station serving Limerick city and county
 * Rickettsia-like organisms (RLOs)