S27

S27 may refer to:

Aviation

 * Blériot-SPAD S.27, a French biplane airliner
 * Kalispell City Airport, in Flathead County, Montana, United States
 * Short S.27, a British military trainer
 * Sikorsky S-27, a Russian biplane bomber

Rail and transit

 * S27 (Long Island bus)
 * S27 (Munich), a line of the Munich S-Bahn
 * S27 (Südostbahn), a railway service in Switzerland
 * Rankoshi Station, in Hokkaido, Japan

Other uses

 * 40S ribosomal protein S27
 * British NVC community S27, a swamps and tall-herb fens community in the British National Vegetation Classification system
 * County Route S27 (California)
 * HMS Revenge (S27), a submarine of the Royal Navy
 * S27: Take off immediately all contaminated clothing, a safety phrase
 * Sulfur-27, an isotope of sulfur
 * USS S-27 (SS-132), a submarine of the United States Navy