Swimming at the 2001 World Aquatics Championships – Women's 4 × 100 metre freestyle relay

The women's 4 × 100 metre freestyle relay event at the 2001 World Aquatics Championships took place in Marine Messe in Fukuoka, Japan in 23 July 2001.

At the start of the event, the World (WR) and Championship (CR) records were:
 * WR: 3:36.61 swum by United States on September 16, 2000 in Sydney, Australia.
 * CR: 3:37.91 swum by China on September 7, 1994 in Rome, Italy.