Swimming at the 2007 World Aquatics Championships – Women's 50 metre butterfly

The Women's 50m Butterfly at the 2007 World Aquatics Championships took place on 30 March (prelims & semifinals) and the evening of 31 March (finals) at the Rod Laver Arena in Melbourne, Australia. 99 swimmers were entered in the event, of whom 93 swam.

Existing records at the start of the event were:
 * World Record (WR): 25.57, Anna-Karin Kammerling (Sweden), 30 July 2002 in Berlin, Germany.
 * Championship Record (CR): 25.84, Inge de Bruijn (Netherlands), Barcelona 2003 (26 July 2003)