Talk:16S ribosomal RNA

27F
Please note, the sequence shown for primer 27F does not agree with that shown for Ref [13]. Which is correct?
 * 27F sequence was fixed (deleted G) — Preceding unsigned comment added by 150.29.67.187 (talk) 22:47, 20 August 2019 (UTC)

Genes VIII
The introduction refers to "Genes VIII p 160". If this is a reference work it should at least be in the references. Wppds (talk) 09:12, 5 January 2009 (UTC)
 * Removed. --Arcadian (talk) 14:46, 5 January 2009 (UTC)

A red link is created based on the following....
--222.67.216.56 (talk) 02:24, 26 August 2009 (UTC)
 * http://scholar.google.com/scholar?as_q=microbial+identification&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en
 * Please see WP:ALSO which explains that "see also" is for further information of direct relevance to the article (with exceptions as required). In particular, a red link is never helpful and should not be used in "see also". Where it really is appropriate, it is good to include sourced information in the article, with red links in that information. Johnuniq (talk) 04:31, 26 August 2009 (UTC)

More on structure etc
I've made some notes (including figures) on the structure of 16S on my blog, mainly aggregating stuff that's not covered in the article. I'm not going to link to my own blog from WP, but if somebody else thinks it's useful, please go ahead. (Unfortunately, the figures are ripped from various places, so they can't be used directly by WP). exploring 16s Ketil (talk) 11:07, 22 September 2010 (UTC)

Dead link
During several automated bot runs the following external link was found to be unavailable. Please check if the link is in fact down and fix or remove it in that case!


 * http://aem.asm.org/cgi/pmidlookup?view=long&pmid=17071787}}
 * In 16S ribosomal RNA on 2011-05-25 01:54:45, 404 Not Found
 * In 16S ribosomal RNA on 2011-06-01 19:11:06, 404 Not Found

--JeffGBot (talk) 19:11, 1 June 2011 (UTC)

Dead link 2
During several automated bot runs the following external link was found to be unavailable. Please check if the link is in fact down and fix or remove it in that case!


 * http://dx.doi.org/10.1016/S0378-1097(03)00717-1}}
 * In 16S ribosomal RNA on 2011-05-25 01:54:40, 404 Not Found
 * In 16S ribosomal RNA on 2011-06-01 19:11:36, 404 Not Found

--JeffGBot (talk) 19:11, 1 June 2011 (UTC)

Dead link 3
During several automated bot runs the following external link was found to be unavailable. Please check if the link is in fact down and fix or remove it in that case!


 * http://dx.doi.org/10.1038/417063a}}
 * In 16S ribosomal RNA on 2011-05-25 01:54:40, 404 Not Found
 * In 16S ribosomal RNA on 2011-06-01 19:11:39, 404 Not Found

--JeffGBot (talk) 19:11, 1 June 2011 (UTC)

Perhaps this is what you want. https://www.nature.com/articles/417063a

Arydberg (talk) 03:18, 19 November 2018 (UTC)

Non-standard nucleotides
''Often 8F is used rather than 27F. The two primers are almost identical, but 27F has an M instead of a C. AGAGTTTGATCMTGGCTCAG compared with 8F.''

This is very confusing since I am under the impression that M means A or C. It's somewhat nonsensical to say that 27F has an "A or C" instead of a C. Unless it's a mixture of two different primers for no apparent reason? The source is no longer available. Baratron (talk) 18:01, 3 April 2020 (UTC)
 * I think it does mean a mixture of two different primers, as this is a primer used to amplify many kinds of bacteria and archaea, so it may be useful to have two different primers to match different organisms from environmental samples. Hanif Al Husaini (talk) 05:11, 30 January 2023 (UTC)

Microbiome Not Mentioned
There is content missing from this article. Despite the term microbiome being in the title of two of the sources, neither microbiome nor microbiota are used anywhere in the body of the article. 16s rRNA is the most utilized gene for identification of bacteria in microbiotas' and should be regarded as valuable technology in association to these specific terms (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5535273/, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC523561/#:~:text=The%20part%20of%20the%20DNA,52%2C%2064%2C%20101).

Sdog422 (talk) 20:51, 27 August 2023 (UTC)

Move discussion in progress
There is a move discussion in progress on Talk:16S rRNA which affects this page. Please participate on that page and not in this talk page section. Thank you. —RMCD bot 01:19, 14 September 2023 (UTC)
 * - now closed (as redirect) 3 October 2023
 * 2A04:B2C2:1002:6100:D43E:4654:400:ADE3 (talk) 15:29, 13 March 2024 (UTC)