Talk:Agarose gel electrophoresis

Redundant sections on nucleic acids
Now that this article has been recreated, I'd like to point out that the sections "Conformation of DNA" and "Visualization" are now redundant with the text in Gel electrophoresis of nucleic acids, where they were moved when this article was originally merged. The Visualization section has been improved since then. Since the content of these sections apply equally to agarose and polyacrylamide electrophoresis, I suggest that these two sections be reduced to summaries here (with Hzh's improvements to the Conformation section being integrated with the nucleic acids article) to reduce the redundancy between the articles. Antony–22 (talk⁄contribs) 01:58, 3 July 2013 (UTC)


 * I'm still going through the process of editing it which may take many days, so perhaps wait till it is reasonably completed before we removed sections? I'm still considering how to organize the various articles, some of the sections may be moved, deleted or significantly reduced when I am done with it.  Note however that there isn't really a need to move everything away from a page because the point of having a page on agarose gel electrophoresis is so that all the main relevant information can be found in a single page without going to other pages, but further detailed informationcan be found in other pages should the reader need to know more.    Hzh (talk) 02:57, 3 July 2013 (UTC)


 * I'd noticed that you were still in the process of editing the article, which is why I posted a note here. I'm happy to sit tight until you're done, it's looking good so far.  Antony–22 (talk⁄contribs) 03:42, 3 July 2013 (UTC)


 * Just adding a note that I may put things here which would be moved to other pages later. This is just for simplicity sake, also so that I can gauge later how to organize the content. Hzh (talk) 13:32, 3 July 2013 (UTC)

Reorganization
I am almost done for the time being, so it's time to think about reorganization. I think I will copy the section on migration of nucleic acids (minus a couple of subsections) to replace the corresponding one in Gel electrophoresis of nucleic acids (Factor affecting migration), and reduce the section here. I'm not sure what to do with the subsection on Mechanism of migration and separation, it could go to the Physical basis part of Gel electrophoresis, although part of it is more suitable to the DNA electrophoresis page (so we could have variations of the same topic on both pages, but it will need a little rewriting to suit the gel electrophoresis page). Suggestions on how to organize it would be welcomed. Also if someone can run a few gels of markers at different gel concentrations (0.5%, 1%, 2%) and put them together as a single figure that would be great. I have done it before but the gel photos have faded a lot and I don't do gel electrophoresis now.

There are minor things that needs to be fixed, and I will probably add a few other things later, but I'll leave the bulk to others to expand for the time being. Whether the part on buffers should be expanded, rewritten, or deleted I'll leave it to others. Hzh (talk) 20:28, 6 July 2013 (UTC)


 * For now, it's finished. The section on migration of nucleic acids can be shrunk a little more because most of the content have been copied over to the Gel electrophoresis of nucleic acids page, and the section on general procedure can be expanded.  There can be a small section on its use on protein electrophoresis, also other methodologies.  The applications section can also be expanded.  Faskal had suggested other things to put in this page, so I'll leave those to him.  I think the Gel electrophoresis page needs some work on its theory part, but I probably can't do that now.  I'll probably come back to this and other issues another time. Hzh (talk) 19:19, 8 July 2013 (UTC)


 * It looks really good! I think that the nucleic acid migration section could be reduced a bit more, but overall it's a fine article.  Thanks for fixing it up! Antony–22 (talk⁄contribs) 05:10, 9 July 2013 (UTC)


 * You are welcome. I'll probably trim the migration section another time, sometimes it's clearer how to trim it after leaving it for some time and coming back with a fresh eye, or someone else can do it.  The important thing is to leave all the salient points intact and trim the unnecessary verbiage.  The mechanism subsection can be reduced to just one paragraph, but I think I'll leave it as it is for the time being.  It can be copied over to the Gel electrophoresis page but it would need an extra paragraph on protein. Hzh (talk) 10:47, 9 July 2013 (UTC)

Annie Wang.3643 (Fall 2014): Another application for an agarose gel is to purify the DNA obtained for subcloning. This page could also include a link to the "Gel extraction" page. — Preceding unsigned comment added by Wang.3643 (talk • contribs) 20:28, 1 October 2014 (UTC)

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