Talk:Bacteriophage MS2

Untitled
Article created as an historical milestone in the sequencing of genomes. Pvosta 20:34, 29 November 2006 (UTC)

MS2 in RNA studies?
MS2 protein is used to purify RNA-containing complexes, it seems... [Jurica, 2002, RNA 8:426-429]. Is the MS2 protein from the MS2 bacteriophage? Ta.

Correction to MS2 page
MS2, which infects E. coli, is a different phage than M2 of B. subtilis. I deleted the sentence saying that these were different names for the same bacteriophage. In addition to the difference in bacterial host, MS2 is an RNA phage while the M2 capsid contains a DNA genome. I think that phage M2 may now be called M2Y. Siegele (talk) 22:23, 10 June 2008 (UTC)

Genus
I've introduced the correct genus name (Levivirus) to the box on taxonomy of this virus. See the Wikipedia article on Leviviridae, and also http://phene.cpmc.columbia.edu/ICTVdB/00.037.0.01.001.htm. Richard Lugg (talk) 16:25, 15 November 2009 (UTC)

genes
"It encodes just four genes: the maturation protein (A-protein), the lysis protein, and the replicase protein." That's the maturation protein (A-protein), the coat protein, the lysis protein, and the replicase protein. It would probably be worth mentioning that the replicase enzyme consists of the replicase protein plus 3 E. coli proteins, ribosomal protein S1, elongation factor Tu, & elongation factor Ts. 69.72.27.161 (talk) 23:45, 21 February 2013 (UTC)

Also, there seems to be a problem regarding the size of genes and size of the genome, even when considering full overlap of the lys genes, the sum of the genes is still larger than the 3569 nt genome. I could find mat=1,182; cp=393; lys=228 (with only 33 nt not overlapping other genes); and rep=1,638. These correspond to the coding regions of the proteins as seen on NCBI nucleotides (the links provided in the table).

Jonatp — Preceding unsigned comment added by Jonatp (talk • contribs) 15:54, 16 April 2018 (UTC)

"Levivirus" is obsolete
The article uses the name Levivirus twice. According to the article Emesvirus (and Levivirus) this name is obsolete. Is it OK to just replace it with Emesvirus? Ettrig (talk) 08:32, 18 June 2023 (UTC)

positive-stranded?
The article says: The positive-stranded RNA genome serves as a messenger RNA. The term positive-stranded is new to me. But reading the article about this, my impression is that it means that the strand can be translated. In that case this sentence is very peculiar. Can I change it to say: The whole MS2 genome is one RNA strand that can be directly translated by a ribosome. Also, since the article about positive sense is rather difficult to read, the meaning should be made explicit in the intro. Ettrig (talk) 21:37, 18 June 2023 (UTC)

F factor or F protein?
I asked ChatGPT (4) and it says, slightly edited:

The Life cycle paragraph begins with a factual error: MS2 infects Escherichia coli (a species of enteric bacteria), but the infectivity of this phage is not reliant on the presence of the fertility factor (F factor) in the bacterial cells. The F factor is a plasmid that does indeed provide a mechanism for DNA transfer (conjugation) between bacterial cells via the formation of a pilus, but MS2 doesn't use this pilus as a receptor. Instead, MS2 targets a protein present on the bacterial surface called the F-pilus protein (specifically, the maturation protein binds to a bacterial protein called F-pilin).

This sound reasonable. ChatGPT almost always does. But I don't know how to check what is right. Ettrig (talk) 09:00, 22 June 2023 (UTC)