Talk:Cell-free system

Untitled
suggest merging with cell-free protein synthesis Kgknapp (talk) 07:38, 2 June 2008 (UTC)
 * I'd suggest the other way--this article is more general and could use more information. &mdash; Scientizzle 15:43, 2 June 2008 (UTC)

Wiki Education Foundation-supported course assignment
This article is or was the subject of a Wiki Education Foundation-supported course assignment. Further details are available on the course page. Student editor(s): Jdogzz. Peer reviewers: Suhaninagpal.

Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT (talk) 17:03, 16 January 2022 (UTC)

Suggestions
The article looks to be complete to me. All I can suggest to this is perhaps include a See also for cell-free protein synthesis. M bartolo (talk) 16:26, 25 October 2017 (UTC)
 * I welcome others to mention if the inclusion of the Cell-free protein synthesis link in a See Also section is merited, but per the Wikipedia style guide it probably is unnecessary since I already used it as a Main article reference in my subsection. Thanks for the suggestion, of course!-Jdogzz (talk) 20:52, 25 October 2017 (UTC)

The phrase "mRNA template" is ambiguous - do you mean the mRNA, or the DNA template for the mRNA? Also, what are "tightly coupled ribosomes"? The phrase "12 H2 has been produced per glucose unit of polysaccharides and water" is difficult to understand - you could clarify by writing the chemical equation for this reaction, or defining the molecular nature of the polysaccharide and giving the stoichiometry of it and that of water. Please check the grammar of the sentence "More recent studies..." What is the "glycolytic network extract" - a cellular extract that was somehow purified to contain primarily enzymes from the glycolytic pathway? - this should be clarified to make this interesting sentence understandable. Ireneachen (talk) 17:10, 27 October 2017 (UTC)
 * My understanding of the use of mRNA in the translation process, which was the focus of that experiment, is it reads the mRNA to produce a given product, and I believe that's their meaning here. The paper doesn't lead me to think that they were doing anything other than the usual process of translation, and the mRNA that was being read was the part that was being degraded, but they only use the phrase template twice, once in their abstract and once in their discussion, so it's possible there is a less straightforward interpretation.
 * I have added an aside explaining the meaning of tightly-coupled ribosomes, included a reference to another paper mentioning what they are, and included a red link since the topic isn't currently documented on Wikipedia.
 * The part about the 12 H2, added by a previous author of the Wikipedia page, was also unclear to me at first, but the paper it came from, as well as references within that paper, use the lack of units (mole, gram, etc.) intentionally, it would seem, since the units cancel as seen in the chemical reaction. I thus assumed this was the correct way of presenting the quantity in the literature, but I have added the chemical equation and an explanatory comment of what was new.
 * Interestingly, the grammar was fine, but I missed the original point of the sentence I had meant to write, and that's now been added in.
 * I have clarified what is meant by the glycolytic network extract, though the original paper is not especially clear about the contents of the extract beyond what is now mentioned here in the Wikipedia article.-Jdogzz (talk) 22:25, 27 October 2017 (UTC)

Suggestions for the article
The article is informative and the flow is really good. I think the information on different classes is a bit redundant. As you mention this classification twice before preparation section. I would suggest that you mention about the classification in the 'Type' section which will become an elaborate section, so basically removing the lines from the first paragraph to 'Type'. In the first para maybe you can mention some general scheme about the design and its importance in synthetic biology.(This might help: http://www.sciencedirect.com/science/article/pii/S2405805X1730008X). I really like the 'Uses' section. Suhaninagpal (talk) 21:41, 25 October 2017 (UTC)Suhaninagpal
 * Thanks for the suggestions, I've now removed the redundancies and included a comment on the synthetic biology aspect. Let me know if there's something more specific you had in mind about that point.-Jdogzz (talk) 17:11, 27 October 2017 (UTC)