Talk:Cy3 and Cy5

I started editing this page because I found references to Cy3 and Cy5 in a text book and then in the literature. It is incredibly hard to find the structure or a description of what these are on the web using Google.

After thinking about it overnight I agree that this entire page would be better merged with the cyanine page. If I do not hear otherwise I will do that in 48 hours, at 12:01, 9 December (UTC).

Nwbeeson 12:41, 7 December 2006 (UTC)


 * Thanks Nwbeeson for the structures. I put that request for merging. Go ahead and merge them. --Kupirijo 15:06, 7 December 2006 (UTC)

These are very important in micforarray technologies. I do not how to draw a picture of chemical structure, can someone help?

I just drew them myself here at home, after spending *two* hours using the resources of the University of Michigan Library finding the structure!! Wow! It was really hard to find. I have added it to the article, with a comment. Nwbeeson 23:55, 6 December 2006 (UTC)

"comments posted" - from article page 26.11.06
Product data sheet I have here says cy3 absorbs at 548 and emits at 562 cy5 650, 670. Slightly different than what is listed above.

R> Nothing to worry about. The numbers listed above are the maximamal exitation wavelenghts, the peak in a graph is were the gradient is reversed, the nearby points though have a gradient that is nearly zero so any of the them could be considered the maxiumal value. Furthermore 2nm is a neglegeble difference, such a change in wavelength could be easily due to the fact if it is bound to DNA or to the NHS-ester, even a change of solvent (water or DMSO) could be the cause.