Talk:Directed evolution

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This article is or was the subject of a Wiki Education Foundation-supported course assignment. Further details are available on the course page. Peer reviewers: Aarankin.

Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT (talk) 20:07, 17 January 2022 (UTC)

Untitled
I just saw the last revision to this page. It is true that Arnold's group is a leader in this field, but don't know whether they were the "pioneers" - a reference would be good for this statement.. O Twist 03:21, 1 February 2007 (UTC)

I am quite certain they were not the pioneers in this field... I am going to remove the statement.

Wiki Education Foundation-supported course assignment
This article is or was the subject of a Wiki Education Foundation-supported course assignment. Further details are available on the course page. Student editor(s): Icho0001.

Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT (talk) 19:33, 16 January 2022 (UTC)

Disambiguation page may be necessary
 Discussion on the use of the term 'directed evolution' in transhumanism was moved to the newly created page - see Talk:Directed_evolution_(transhumanism)

Update
Made seveal expansions and added a set of new images. Have updated the quality to level B. Let me know if you think it should be different. T. Shafee (Evo&#38;Evo) (talk) 07:44, 21 December 2014 (UTC)

Life extension section removed
For the moment, I've removed the section on life extension (quoted below) since it is currently speculation on experiments that haven't been done yet in a blog. ===Life extension research=== Researcher Maria Konovalenko, from the Moscow Institute of Physics and Technology, claims that directed evolution can be used to create strands of bacteria that extend the life of their host, and this research may further be used to develop human longevity treatments. If there are any peer-reviewed papers to reference we can integrate it back in. T. Shafee (Evo&#38;Evo) (talk) 08:19, 9 January 2015 (UTC)

Intended updates
I'm hoping to do some updates over the coming months. Unstructured ideas below (feel free to add)

Images

 * File:DE cycle.png could do with being updated to emphasise the proteins, rather than cells - more like this example?
 * Would any additional diagrams be useful?

Sections
T.Shafee(Evo &#38; Evo)talk 11:28, 4 December 2018 (UTC)
 * The "principles" section could be updated and expanded.
 * The "combinatorial approaches" subsection is probably in need or expansion since this is increasingly common
 * inc. description of focussed / hotspot mutagenesis here or in "generating variation" section
 * Add an "examples" sub section as part of the applications heading so writing up a few illustrative examples concisely will be useful (e.g. phage display probably the most common)
 * Add some sort of "common outcomes" section describing typical features
 * enhancement increases and diminishing returns
 * getting trapped in local optima
 * common mutation locations
 * stability/activity tradeoffs
 * promiscuous activity fluctuations
 * Need to mention continuous evo somewhere, possibly as part of "selection" section
 * Should clarify big difference in selection for binding vs catalysis

Question in Generating variation section
Hi, I had a question on the mentioned section. when you sai "The sequence space for random sequence is vast (10^130 possible sequences for a 100 amino acid protein)[...]" Why is that? Natural amino acid are 20, so why not 10^130? hoping to be useful, Best regards Faballi (talk) 13:22, 3 August 2022 (UTC)