Talk:Epitope mapping

Another method
[http://www.genengnews.com/gen-articles/mapping-epitopes-with-h-d-ex-mass-spec/3663/ Mapping Epitopes with H/D-Ex Mass Spec. May 2011] describes using deuteration followed by mass-spec to map at medium resolution (10-15 residues). "Some limitations to H/D-Ex include heavily glycosylated proteins in which it may be difficult to identify glycosylated peptides after digestion, and heavily di-sulfide-bonded proteins, which are difficult to digest and make it difficult to identify peptic fragments. Also, proteins larger than 100 kDa may have reduced sequence coverage." - Rod57 (talk) 06:13, 17 May 2011 (UTC)