Talk:Explant culture

Wiki Education Foundation-supported course assignment
This article is or was the subject of a Wiki Education Foundation-supported course assignment. Further details are available on the course page. Student editor(s): Untediousness.

Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT (talk) 21:03, 16 January 2022 (UTC)

Assessment comment
Hello, I am planning to organize and add on more details to this article. These are the proposed additions I am trying to make, and the references are attached after the outline.

Introduction: Add proper references so readers are able to take a look at primary or even secondary literature. Take out more specific details that are too much for introduction and relocate it to different subsections. Discuss advantages of explant culture in terms of applications.

Applications: Developmental biology – Explain how the field is currently studying morphogenesis and organogenesis with explant culture. Go over how chemical impact of experimental molecule can be studied and gene expression can be observed with aide of fluorescence microscopy. Organ engineering - Talk about how induced pluripotent cell explant culture can be fully grown in vitro and transplanted to a patient; currently several simpler organs such as bladder, trachea, skin and bone marrow have been generated—most promising organ seems to be liver. Discuss difficulties and the impact based on technique’s success.

Protocol: Describe most general methods researchers in the field are using to grow explant culture. There is a consensus on what preparation controls should be used for calibrations and caveats for using explant protocols should be noted.

Current Research: Bone and Cartilage – The model developed overcomes the problem of scarcity of healthy human bone sample. Explain how using animal tissue explants can be helpful to understand behavior of cells going through development, metabolism, and repair. Retina – Retinal tissues from rats are currently being studied to study neurodegeneration and neuroprotection. Explain how the study can help developing new therapies. Heart – An explant of neonatal rat ventricle has been proposed to be a promising model to study gene and cell therapy. Explain the potential of the model, which hope to focus on therapies for cardiovascular diseases.

bibliographies:

Haan, A. DÃ©nise Den, Marieke W. Veldkamp, Diane Bakker, Geert J. J. Boink, Rob B. Janssen, Jacques M. T. De Bakker, and Hanno L. Tan. "Organ Explant Culture of Neonatal Rat Ventricles: A New Model to Study Gene and Cell Therapy." PLoS ONE 8.3 (2013): n. pag. Web.

Johnson, Thomas V., and Keith R. Martin. "Development and Characterization of an Adult Retinal Explant Organotypic Tissue Culture System as an In Vitro Intraocular Stem Cell Transplantation Model." Investigative Opthalmology & Visual Science 49.8 (2008): 3503. Web.

JoVE Science Education Database. Essentials of Developmental Biology. Explant Culture for Developmental Studies. JoVE, Cambridge, MA, (2017).

Marino, Silvia, Katherine Ann Staines, Genevieve Brown, Rachel Anne Howard-Jones, and Magdalena Adamczyk. "Models of Ex Vivo Explant Cultures: Applications in Bone Research." BoneKEy Reports 5 (2016): n. pag. Web.

Pathak, Meeta, O.k. Olstad, Liv Drolsum, Morten C. Moe, Natalia Smorodinova, Sarka Kalasova, Katerina Jirsova, BjÃ¸rn Nicolaissen, and Agate Noer. "The Effect of Culture Medium and Carrier on Explant Culture of Human Limbal Epithelium: A Comparison of Ultrastructure, Keratin Profile and Gene Expression." Experimental Eye Research 153 (2016): 122-32. Web.

(talk) 21:54, 11 May 2017 (UTC)UntediousnessUntediousness (talk) 21:54, 11 May 2017 (UTC)

I am planning to add some more details from developmental biology perspective and possibly info from protocols that are widely used to make explant culture. Are there any other directions that I should think about? I am taking suggestions.Untediousness (talk) 22:27, 4 May 2017 (UTC)

Substituted at 14:51, 29 April 2016 (UTC)