Talk:Expressed sequence tag

Nice
It seems to me that DNA microarrays are used EVALUATE COMPARATIVE diffences in gene TRANSCRIPTION LEVELS, and not to DETERMINE GENE EXPRESSION. The nuance may be subtle... Also, it would be a good idea to explain from where and when a value of 6 million known ESTs as been approximated.

Another topic of interest : Being derived from mRNAs by retro-transcription, are poly-A primers used or random ones? Does it mean that there can be introns in the sequences (as if the cDNAs were obtained from non-spliced mRNAs) as well as 3' and 5' untranslated regions?

Very good article. Marooned Morlock (talk | contribs) 08:48, 11 May 2006

Patents on EST's
Does anyone have any thoughts on adding to this article information on patent requests on EST's by various BioTech companies? I do recall a story done on a major TV newsmagazine, possibly 20/20 or 60 Minutes, but it was some time ago and I haven't heard much from the mainstream news recently on this. Google produces a significant number of results for the search +patent +gene, but is there enough referenceable info to warrant a separate article for this topic? Ipso-De-Facto 02:34, 9 October 2006 (UTC)

???
If you don't know the technique, you won't understand it by reading this article. --85.179.34.104 (talk) 11:09, 24 September 2008 (UTC)

Page needs updating
There's still a section stating "as of 2006", surely this means the page needs a freshening up? — Preceding unsigned comment added by Louismaddox (talk • contribs) 05:07, 13 January 2015 (UTC)

External links modified
Hello fellow Wikipedians,

I have just added archive links to 6 one external links on Expressed sequence tag. Please take a moment to review my edit. If necessary, add after the link to keep me from modifying it. Alternatively, you can add to keep me off the page altogether. I made the following changes:
 * Added archive http://web.archive.org/web/20070228181445/http://www.ncbi.nlm.nih.gov:80/About/primer/est.html to http://www.ncbi.nlm.nih.gov/About/primer/est.html
 * Added archive http://web.archive.org/web/20070829194022/http://www.biolinfo.org/EST/ to http://www.sciencemag.org/cgi/pmidlookup?view=long&pmid=2047873
 * Added archive http://web.archive.org/web/20080604225500/http://icb.med.cornell.edu:80/crt/tissueinfo/ to http://icb.med.cornell.edu/crt/tissueinfo/
 * Added archive http://web.archive.org/web/20070228181445/http://www.ncbi.nlm.nih.gov:80/About/primer/est.html to http://www.ncbi.nlm.nih.gov/About/primer/est.html
 * Added archive http://web.archive.org/web/20070829194022/http://www.biolinfo.org/EST/ to http://biolinfo.org/EST/
 * Added archive http://web.archive.org/web/20080604225500/http://icb.med.cornell.edu:80/crt/tissueinfo/ to http://icb.med.cornell.edu/crt/tissueinfo/

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Cheers.—cyberbot II  Talk to my owner :Online 11:09, 28 February 2016 (UTC)

External links modified
Hello fellow Wikipedians,

I have just added archive links to 1 one external link on Expressed sequence tag. Please take a moment to review my edit. If necessary, add after the link to keep me from modifying it. Alternatively, you can add to keep me off the page altogether. I made the following changes:
 * Added archive http://web.archive.org/web/20080604225500/http://icb.med.cornell.edu:80/crt/tissueinfo/ to http://icb.med.cornell.edu/crt/tissueinfo/

When you have finished reviewing my changes, please set the checked parameter below to true or failed to let others know (documentation at ).

Cheers.—cyberbot II  Talk to my owner :Online 02:40, 19 March 2016 (UTC)