Talk:Gamma-butyrobetaine dioxygenase/GA1

GA Review
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Reviewer: Sasata (talk · contribs) 21:02, 30 September 2013 (UTC)

Hi, I'll review this article. Will have comments up in a few days. Sasata (talk) 21:02, 30 September 2013 (UTC)

Comments
 * lead: suggested links – activated fatty acid, mitochondrial membrane, beta-oxidation, expression, microarray
 * Done. Adabow (talk) 22:40, 7 October 2013 (UTC)


 * the enzyme infobox is in an unfortunate location, squeezing the accompanying text, which makes the biochemical reaction harder to read; I suggest moving this info box to the right hand side underneath the main infobox
 * could we have a little more background about the significance of the reaction; it's not until later we learn that the enzyme is involved in "lysine degradation", but I think this should be made explicit earlier
 * "Similar to many other 2OG oxygenases, the activity of gamma-butyrobetaine dioxygenase can be stimulated by reducing agents such as ascorbate and glutathione.[7][8][9][10]" why are four sources needed for this statement?
 * "The catalytic activity of gamma-butyrobetaine dioxygenase can be stimulated with different metal ions, especially potassium ions." this is sourced to a paper from 1988; in the intervening time, has there been any research into the nature of this stimulatory effect?
 * "Both the apo (PDB id: 3N6W)" for a general encyclopaedia article, one should assume the reader will not know biochemical jargon, so PDB should be spelled out and linked on 1st occurrence
 * "demonstrating an induced fit mechanism may contribute to the catalytic activity of gamma-butyrobetaine dioxygenase." source?
 * "promiscuis" spell correctly and link; also link substrate selectivity
 * Promiscuous done; no WP article for substrate selectivity. Adabow (talk) 22:40, 7 October 2013 (UTC)


 * "natural catalytic product L-carnitine and D-carnitine" products
 * check article for repeated links
 * "Some studies suggested that mildronate may also be beneficial for the treatment of neurological disorder,[22][23] diabetes,[24] and seizures and alcohol intoxication.[25]" this is a sweeping generalization; is this MEDRS compliant?
 * "To date, at least five clinical trial reports were published in peer-reviewed journals documenting the efficacy and safety of mildronate on the treatments of angina, stroke and chronic heart failure.[26][27][28][29][30]" I don't think these citations are necessary or appropriate for an article on the enzyme; is there a secondary source which summarizes these reports?
 * link binding pocket, non-competitive
 * Done. Adabow (talk) 22:40, 7 October 2013 (UTC)


 * where's the history? Who first reported the enzyme? The article only talks about the human version of the enzyme. Has it been reported in other mammals? How close are the sequences?
 * the article states the enzyme structure has been determined, but there is no discussion about its structure. Is is monomeric or multimeric? Any conformational changes with binding? Is it a soluble enzyme or located in the membrane? What is its MW? Isoelectric point?
 * the last sentence of the lead mentions that "BBOX1 has recently been identified as a potential cancer gene on the basis of a large-scale microarray data analysis." but this is not discussed anywhere else (a violation of WP:LEAD); please give details
 * Not much movement on this GAN, so I'm closing it. Hopefully these comments will be useful to other editors who wish to improve the article. Sasata (talk) 17:32, 14 October 2013 (UTC)