Talk:Heterochromatin protein 1

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"HP1 was originally discovered by Yani Zhou in 1986 as an important factor in the phenomenon known as position effect variegation in Drosophila melanogaster" I am not aware of this contribution. T. C. James--Jthrppel (talk) 11:19, 28 January 2011 (UTC)

I noticed this page is lacking a mechanism section, I'd like to add to that.

This article has some good information on the function of the CD as part of HP1. Though, I do agree a simple search is hard to find the precise function of HP1 in simple language.

https://www.researchgate.net/publication/12362669_Conservation_of_Heterochromatin_Protein_1_Function

--GTF17 (talk) 13:03, 8 December 2017 (UTC)

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Something ios wrong with the hyperlink to Reference 1 The link to the DOI is dead but the DOI itself is correct DOI: 10.1128/mcb.6.11.3862 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC367149/

Wiki Education Foundation-supported course assignment
This article was the subject of a Wiki Education Foundation-supported course assignment, between 24 August 2020 and 11 December 2020. Further details are available on the course page. Student editor(s): Gdpratt.

Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT (talk) 09:33, 18 January 2022 (UTC)

Edit discovery of HP1
Note that HP1 was originally discovered based on a cytological assay. TC James prepared monoclonal antibodies against proteins tightly bound in the nuclei of Drosophila melanogaster embryos, and used these to stain the polytene chromosomes from third instar larvae. An antibody that showed high concentration in the chromocenter/pericentric heterochromatin, specific to a single nuclear protein by Western blot, was used to screen an expression library, recovering a cDNA clone. This enabled both a preliminary determination of the amino acid sequence and identification of the genetic locus as 29A on the second chromosome (James and Elgin, 1986). Both Thomas Grigliatti (University of British Columbia) and Guntur Reuter (Halle University, Germany) had previously identified Suppressor of Variegation mutants in this region, and kindly supplied the mutant lines. Sequencing of a mutant line from Grigliatti showed that a mutation in the gene for HP1 indeed led to a loss of heterochromatin silencing, indicating the functional role of HP1 (Eissenberg et al. 1990, Proc Natl Acad Science USA 87, pp. 9923-27).Carlisle1953 (talk) 21:56, 16 March 2022 (UTC)