Talk:Holliday junction/GA2

GA Review
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Reviewer: Chiswick Chap (talk · contribs) 12:57, 22 May 2015 (UTC)

This seems an interesting and well-written article; I'll take it on. Chiswick Chap (talk) 12:57, 22 May 2015 (UTC)

Comments
The lead needs to be extended to reflect the article's contents. For example, the structure's involvement in double-strand breaks is important and certainly deserves a paragraph in the lead. As general guidance, the lead should summarize every section and major theme of an article. This is the main item (now that I've checked through the article); but it shouldn't take too long to draft a suitable lead.

History: "..., with Robin Holliday himself initially doubting the findings." Best to avoid this construction with -ing: try "...; Holliday himself initially doubted the result."

"copy-choice mechanism" is redlinked: it needs a word of explanation here, whether or not you choose to write a short article on that topic.

Use in DNA nanotechnology:
 * tensegrity is linked but needs a brief gloss here.
 * Also, the caption of the "Diagram of a tensegrity triangle complex" doesn't really explain what a, b, and c actually are.
 * I can see there's an angstrom scale but even that really needs explaining wrt SI units (nm). Or wikilinking.

Structure: please wikilink mM (milliMole/liter, I presume?).

"RNA Holliday junctions are known in some functional biological RNAs": it may be helpful here to explain briefly what these RNAs do, and why having a Holliday junction helps with that function, so readers can connect structure with function. I can see that this might be little more than "This gives the molecule such-and-such a shape, which enables it to present a reaction site ...", but even that would make the article easier to read.
 * See below, this item remains open.

"RNA Holliday junctions are..." (cont'd): I also think that this paragraph needs to be related to the "Biological function" section, or might actually be a subsection of it, once beefed up with some functional explanation. That would make "Biological function" a section with 2 (named) subsections, not a bad plan probably.

References: Ref 5 is to a whole book (Hartel 2012): a page ref is needed.

Ref 8 has a Delphic '"43"' - is that a page? chapter?

Chiswick Chap (talk) 12:57, 22 May 2015 (UTC)


 * I believe I've dealt with all the items above, with the exception of the conceptual description of how the four-arm junction structure contributes to RNA function. I don't think that high-level conceptual questions like that have answers that are well understood at this point. Antony–22 (talk⁄contribs) 20:59, 1 June 2015 (UTC)
 * Then we need to say something along the lines of while the HJ influences the shape or conformation of the RNA molecules, its precise effect on function is not yet understood. Otherwise people will be reading the article wondering when it's going to get to the point and explain what the structure is actually for. The least we can do is say plainly that scientists don't know. Chiswick Chap (talk) 06:37, 2 June 2015 (UTC)
 * That's not quite what I meant. Simple structures like this don't have a "precise effect on function"; the function is the result of the conformation of the molecule as a whole.  It's kind of like asking what is the unifying purpose of circles in automobiles.  The tires, steering wheel, and buttons on the dashboard may all be circular, but there's no consistent conceptual reason for this.  Antony–22 (talk⁄contribs) 20:56, 2 June 2015 (UTC)
 * No, we're wandering off topic. That isn't what I meant at all, but it's tangential to the article, applying really only to the immobile not-quite-HJs that are mentioned briefly. It'll do as it is. Chiswick Chap (talk) 21:35, 2 June 2015 (UTC)