Talk:Immunofluorescence

Wiki Education Foundation-supported course assignment
This article is or was the subject of a Wiki Education Foundation-supported course assignment. Further details are available on the course page. Student editor(s): Herrerais. Peer reviewers: Marissaogata.

Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT (talk) 00:19, 17 January 2022 (UTC)

I
I think this is a fairly important method, and more detail (on fluorophores and microscopy methods in particular) would be helpful. I can do work when I get a chance, but anyone who wants to make a start, feel free! Dr Aaron 04:27, 16 September 2006 (UTC)

I think this article needs to be more connected to the immunohistochemistry article. In many ways they describe the same. --LasseFolkersen (talk) 08:30, 20 February 2008 (UTC)

WikiProject class rating
This article was automatically assessed because at least one WikiProject had rated the article as stub, and the rating on other projects was brought up to Stub class. BetacommandBot 16:28, 10 November 2007 (UTC)

Merge proposal
I am proposing to merge Immunostaining protocol into Immunofluorescence. The two articles are simply too short to splinter into the two acknowledge. ChyranandChloe (talk) 01:41, 20 December 2008 (UTC)

Outline for Improvements
Outline for improvement of the article:

I want to add more about the background of Immunofluorescence mainly bridging the gap between immunohistochemistry and immunofluorescence. The current lead section mentions it as a subclass but does not make it clear how the two ideas are connected. I would also want to include a section called "Super Resolution Methods." Again, the lead paragraph suggests these methods but does not provide an adequate amount of depth in the subject. I want to briefly go into the different super-resolution methods that can be used in relation to immunofluorescence. I also want to add information on the specific fluorophores and the chemistry behind their binding to antibodies used for fluorophores. This also includes going into more specific details about limitations surrounding immunofluorescence in certain applications in both the clinical setting and in research. Herrerais (talk) 05:56, 20 February 2017 (UTC)

I fully agree and I am happy to support the writing.CaC (talk) 10:55, 5 April 2017 (UTC)

Tagged or conjugated
"tagged or conjugated" is used several time in the text. What is the difference? CaC (talk) 10:56, 5 April 2017 (UTC)

What is it for?

 * As a layman, I came to this page looking to learn what this is for, and am finding it hard to figure it out based on what’s here. Could someone who knows add some information clearing up what this is used on (objects or living things? What kinds?) and to what end (academic, commercial, medical or multiple purposes?). For context, I was reading the NIHs’ ‘Autoimmunity may be rising in the United States’ piece. I looked up immunofluorescence expecting it to be something specifically to do with autoimmune diseases in humans, but it’s not clear here if it’s that, or a wider thing, or not to do with that at all. The answer is not important to me. I just feel it would be useful for future lay readers. Any help appreciated!—  A L T E R C A R I  ✍ 02:16, 18 August 2022 (UTC)

Course assignment
hello, I am yasemin alkassem a molecular biology master student, and I am editing this article as a part of my assignment( more details about the course are available on the course page). Yasemin-alkassem (talk) 08:50, 1 January 2023 (UTC)