Talk:Integrase

How does it work?
Hello, Is there a specific DNA sequence where integrase works? Is the viral DNA capable to enter in any site of human genome? — Preceding unsigned comment added by 109.242.44.250 (talk) 23:14, 18 December 2011 (UTC)

is there a difference between HIV and HIV-1 integrase?

HIV has two most common strains HIV-1 and HIV-2 in North America/ Europe and Africa, respectivly.


 * I have no idea, but a good place to take these questions might be Reference Desk. Yours, Meelar 14:59, 1 May 2004 (UTC)

Restructure
Ok this page needs some major revamping....

My current research involves both HIV-1 and RSV integrase. When I have time I'm gonna come in here and write up a nice article.

Image
Hello, does anywhere on the internet exist a 3D image, animation or chemical structure chart of already integrated DNA (with exact molecules/atoms)?

RE: What are you asking here? There is no published data in regards to where integrase is bound to either viral or cellular DNA. Integrated DNA would essentially look like any DNA molecule.


 * I posted a more detailed question at Science section at Reference Desk at Nov.10th 2007. Thanks for any help. I'm just looking for published papers, books or anything covering base pair sequences of already integrated HIV viral DNA around those "attachement" sites. I apologize for my bad English.

Integrons using integrase
Integrons also use integrase to pick up bacterial genes/casettes of genes and recombine them in other situations eg. in plasmids/genomes. Are integrons considered viruses? I would suggest that the field considers integrons as mobile elements in prokaryotic genomes which contribute significantly to their evolution. There's a good review of integrons here : http://www.ncbi.nlm.nih.gov/pubmed/7783631?dopt=Abstract I may do something to this page myself if I have time after revision pet (talk) 00:25, 1 May 2008 (UTC)

Protein Databank and Last Paragraph
The last paragraph says, "Elucidation of the HIV-1 integrase structure has been unsuccessful despite numerous efforts.". However, casually searching the Protein Databank ([www.pdb.org]), I found:


 * HIV INTEGRASE CORE DOMAIN COMPLEXED WITH TETRAPHENYL ARSONIUM (1HYV, 2001)
 * HIV integrase (3LPT, 2010)
 * HIV integrase (3LPU, 2010)

Is this technically correct? --InsufficientData (talk) 01:49, 11 April 2011 (UTC)
 * I skimmed the Nature article. I think the article makes the point that the so-called "intasome" hasn't been solved (the integrase + all the helper proteins it uses to perform its splicing activity).  I'm unsure if the the PDB structures I referenced include the full conformation given all helper enzymes.  There could in theory be an active site change with and without the helper proteins which is significant.  --InsufficientData (talk) 02:07, 11 April 2011 (UTC)