Talk:N1-Methylpseudouridine

Other names
https://www.frontiersin.org/articles/10.3389/fcell.2021.789427/full links to these studies as examples of N1-methyl-Ψ vaccines, but I'm not a biochemist and just want to make sure they really are synonyms:


 * "N1-methyl-Ψ was used and incorporated into two similar mRNA vaccines encoding Zika virus surface proteins. The modified mRNA, encapsulated in LNPs, was designed and then tested to protect against"
 * "the Zika virus in human cells, mice, and non-human primates (Pardi et al., 2017; Richner et al., 2017)."
 * Pardi calls it "1-methylpseudouridine (m1Ψ)"
 * Richner calls it "1-methylpseudoUTP"
 * "In the following year, further success was obtained with N1-methyl-Ψ-modified mRNA vaccines against HIV-1, Zika, and influenza virus, achieving a sustained antibody response in a preclinical setting (Pardi et al., 2018)."
 * Calls it "1-methylpseudouridine" and "m1Ψ"
 * "A similar example was presented against the Ebola virus in guinea pigs (Meyer et al., 2018)."
 * Calls it "1-methylpseudo UTP"

Assuming these really are synonyms for identical molecule, they should be mentioned in the article. — Omegatron (talk) 15:16, 15 January 2022 (UTC)

Wobble-pairing of Ψ but not m1Ψ?
As I put in the article, there are two papers that have contradictory claims about m1ψ wobble-pairing:


 * Xia says:
 * "all U nucleotides were replaced by N1-methylpseudouridine (Ψ) [14,15]. However, Ψ wobbles more in base-pairing than U and can pair not only with A and G, but also, to a lesser extent, with C and U [16]." Source 16 is Kierzek 2014
 * Morais says:
 * "However, Ψ contains an extra hydrogen bond donor group (N1H) that contributes to a universal base character, i.e., it can not only pair A but also wobble base-pair with G, U, or C in the context of a duplex (Kierzek et al., 2014)."
 * "N1-methyl-Ψ can only use its Watson-Crick face to base-pair with another nucleoside, thus preventing it from wobble-pairing with other nucleotides (G, U, and C)." but does not provide a reference for this claim, only references its own Figure 1.

So both reference Kierzek, but for different claims. Kierzek seems to be only about Ψ and not about m1Ψ, which would mean that Xia's claim is incorrectly sourced, and therefore both claims are unsourced? — Omegatron (talk) 16:56, 15 January 2022 (UTC)


 * That is right. Kierzek only talks about Ψ, not m1Ψ. Alfa-ketosav (talk) 07:47, 14 June 2023 (UTC)