Talk:Quantitative polymerase chain reaction

Redirect
I agree with the below, and so I'll just go ahead and make the redirect link for all associated linking pages. Someone needs to. --155.212.249.253 (talk) 17:24, 11 June 2008 (UTC) I forgot to log in. The directly above is me. --Michael (talk) 17:24, 11 June 2008 (UTC)

I agree with the below poster, this should link us to the much larger and better composed real-time PCR page. Real-time PCR and qPCR are synonymous in any usage I've ever heard. —Preceding unsigned comment added by 146.189.246.13 (talk) 15:17, 16 May 2008 (UTC)

I don’t understand the purpose of this article as there is a preexisting article ‘Real-time polymerase chain reaction’. There could simply be a link to the 'real-time PCR' article, which is largely the identical technique. The 'real-time' article could be improved if necessary. This qPCR aritcle also contains ambiguities, grammatical errors, links to commercial products, and is not spell checked.

Some of the problems I noticed:

No explanation of what “real-time” means.

“the temperature-mediated enzyme DNA polymerase”: There are hundreds of DNA polymerases. What does “temperature-mediated” mean? Every enzyme requires a temperature; very few work at abosolute zero.

“The three commonly used methods of quantitative polymerase chain reaction are is through agarose agarose gel electrophoresis, the use of SYBR Green, a double stranded DNA dye, and the fluroscent reporter probe.”: These are not methods of PCR, but modifications to examine the products of PCR.

“Agarose gel electrophoresis is the simplest method, but also the slowest and least accurate,”: Agarose gel electrophoresis is lower tech, but I'm not sure it's the "simplest". It is less quantitative, but it provides more exact data on PCR product size then the other methods.

“3. A Software for SYBR Green primer design is also available. Check Premier BioSoft. [1]” I don’t see why a link to this commercial company is present. There are dozens of different primer design softwares available.

“External Links      * Gene quantification”:  Another heavily commercial link(?). —Preceding unsigned comment added by 66.30.25.2 (talk • contribs)