Talk:Reading frame

Vague statement
"Double stranded DNA has six different reading frames molecule due to the two strands from which transcription is possible - three of them reading forward and three of them reading backwards." Is it correct to interpret this statement as saying: double stranded dna consists of 2 strands with opposite alignment (3' 5' direction is reversed, and reading frame may be on either side, but is always read in the same 3' to 5' or was it opposite direction)? otherwise I see 2(strands)*3(nucleotides)*2directions=12 reading frames... — Preceding unsigned comment added by 213.49.90.22 (talk) 06:04, 27 November 2011 (UTC)
 * Double stranded DNA consists of two strands with opposite alignment (i.e., the strands are antiparallel). Transcription (I think, this could be wrong) only happens in a 3' to 5' direction, or to put it another way, from the hydroxyl end of a DNA strand towards its phosphoryl end. Therefore each DNA strand can only have three possible reading frames (being read in one direction), and therefore double stranded DNA has a total of six reading frames. To make things more confusing, I think that RNA may be able to be read in either direction during translation, I will check this later, and try to remove ambiguity from the article -Zynwyx (talk) 09:57, 29 May 2012 (UTC)
 * The use of "3' to 5' direction" without any prior explanation has to be corrected. What does it mean? Where is this terminology taken from? How is the reader supposed to know? LeadSongDog come howl!  00:58, 20 June 2012 (UTC)
 * I have added a short section in the lede explaining directionality of nucleic acid strands, but it might still be too technical. Also sorry, I think I may have rearranged a couple of your references to the wrong place, please feel free to move them back. I've added that a DNA double helix (technically) has 12 reading frames, but not all of them are biologically relevant. I think this page may need attention from an expert, I am not sure what the consensus is on what counts as a reading frame or not, so am reluctant to make any more edits. Does a reading frame need to be biologically relevant to be counted as such? -Zynwyx (talk) 01:40, 21 June 2012 (UTC)
 * A double helix only has 6 possible reading frames, as each strand can only be read in one direction. If you're going the other direction, you're reading the other strand.  Note that stop codons have no relevance during transcription: in the case of mRNAs, transcription is terminated some distance after the stop codon by a separate nucleic acid sequence, whereas non-coding RNAs don't have stop codons at all.
 * What this and related articles really need is some good, clear diagrams. We could also replace the early refs to papers with refs to a free textbook such as Alberts Molecular Biology of the Cell. 4th ed, available here, which is less technical and better illustrated.
 * Adrian J. Hunter(talk•contribs) 11:06, 21 June 2012 (UTC)
 * This glossary helped. The missing nugget was in the Directionality (molecular biology) article, though still not terribly clear there. The "5 prime end" and "3 prime end" are distinguished by where the phosphoryl or hydroxyl attachments to them are made. The number "5" refers to "which" carbon (the fifth) in the furanose sugar-ring at that head end is phosphorylated or hydroxylated, just as the number "3" refers to the third carbon in the sugar ring at the tail end is hydroxylated or phosphorylated. These carbon positions on the ring are counted from "1" (where the basic group attaches). I hope that's now clarified after my last edit, though it probably still needs more elaboration. LeadSongDog come howl!  18:05, 21 June 2012 (UTC)

Question
According to Lewin's Genes XI, the reading frame should on the mRNA rather than on the DNA. the DNA needn't be divided into the triplets, because the molecule translated is the mRNA rather than DNA.And the enzyme just transcrips the DNA, not divide it. The dividing happens when the ribosome is reading the mRNA. --   パンツァー VI-II  ❂Fu7ラジオ❂In the Republic of China 103rd.民國103年 14:24, 13 November 2015 (UTC)

Assessment comment
Substituted at 04:00, 30 April 2016 (UTC)

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