Talk:Tetracycline-controlled transcriptional activation

Untitled
Tet-on and Tet-off are based on the same concept and not different enough that they require separate pages. I think that one page can handle both with subsections handling their differences.Jvbishop 17:00, 16 February 2007 (UTC)
 * I will merge the pages if no one objects by February 25th 2007Jvbishop 17:03, 16 February 2007 (UTC)

You could also fix some of the fairly weird English of this article. Looks like someone from Heidelberg may have put these up here...Engrish as in English+German that is... —Preceding unsigned comment added by 129.132.210.98 (talk) 08:10, 28 July 2008 (UTC)

is the recent discussion of mito effects relevant to this article?
for example,

http://www.the-scientist.com//?articles.view/articleNo/42414/title/Widely-Used-Antibiotics-Affect-Mitochondria/

he tetracycline-controlled promoter system is a widely used tool to conditionally switch gene transcription on or off in the presence of the eponymous antibiotic. Adding tetracyclines to eukaryotic cells leads to altered mitochondrial genome translation and cellular respiration defects across five widely used eukaryotic model systems—Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, laboratory mice, and human cell lines—according to a study published today (March 12) in Cell Reports.

The results suggest that using this gene expression control system likely has broad confounding effects on experimental outcomes in molecular biology. And with tetracyclines accounting for 41 percent of all antibiotics sold for use on livestock in the United States in 2011, according to the US Food and Drug Administration, environmental accumulation of the drugs could have detrimental ecological outcomes. — Preceding unsigned comment added by 50.180.45.3 (talk) 11:49, 15 March 2015 (UTC)

Tet-ON diagram incorrect?
The image appears not to accurately represent either the Tet-Off or the Tet-On system as described by the text.

According to the text, in the Tet-Off system, tetracycline binds tTA and renders it incapable of binding to TRE sequences, repressing transcription. In the Tet-On system, tetracycline binds rtTA and renders promotes binding to TRE sequences, activating transcription.

The image, however, depicts a system whereby tetracycline binds tTA and renders it incapable of binding to TRE sequences, thus activating transactivation. 129.67.76.158 (talk) 17:32, 22 July 2015 (UTC)

TetO used but not defined
The term TetO is used in the "Tet-Off and Tet-On" section, but it is not previously explained in the article like TetA, TetR, or tTA are. A brief explanation should be given the first time it is used. — Preceding unsigned comment added by Sgrandpre (talk • contribs) 15:20, 13 September 2018 (UTC)