U27

U27 may refer to:

Naval vessels

 * BRAZILIAN TRAINING SHIP Brasil
 * GERMAN SUBMARINE U-27, various vessels
 * HMS Hastings (U27), a sloop of the Royal Navy
 * U-27-class submarine (Austria-Hungary) of the Austro-Hungarian Navy
 * SM U-27, the lead boat of the class

Other uses

 * Cessna U-27 Caravan, an American utility aircraft
 * Rhombicosidodecahedron
 * Small nucleolar RNA SNORD27