USF

USF may refer to:

Universities

 * University of Saint Francis (Indiana), Ft. Wayne, Indiana
 * University of San Francisco, California
 * University of South Florida, Tampa, Florida
 * University of St. Francis, Joliet, Illinois
 * University of Sioux Falls, South Dakota

Locations

 * Universal Studios Florida, a theme park in Orlando, Florida

File formats

 * Universal Subtitle Format
 * Nintendo Ultra 64 Sound Format

Other uses

 * Ukrainian Shooting Federation, national governing body for shooting sports in Ukraine
 * Unmanned Systems Forces, a branch of the Armed Forces of Ukraine for the operation of unmanned equipment
 * Universal Service Fund, a United States government program
 * Upstream stimulatory factor, DNA-binding proteins regulating gene expression, see USF1 and USF2
 * USAfrica Airways, defunct airline