Union Depot and Atlantic Coast Line Freight Station (Live Oak, Florida)

The Union Depot and Atlantic Coast Line Freight Station (also known as the Railroad Passenger Depot and Freight Station) is a historic site in Live Oak, Florida, United States. It is located at 208 North Ohio Avenue, on the corner of Haines Street Northeast. The station was built at one of two junctions of an Atlantic Coast Line Railroad and Seaboard Air Line Railroad lines. It also served the Florida Railway (a line leading to the Mayo area), as well as the Live Oak, Perry and Gulf Railroad.

The station was a flag stop on the SCL and Louisville and Nashville's Gulf Wind, between Madison and Live Oak, and until 1966 an additional daily local train served the station as well. The ACL and the SAL merged in 1967 to form the Seaboard Coast Line Railroad. In 1971 the SCL terminated the Gulf Wind, on the creation of Amtrak, ending passenger service in Live Oak.

On April 24, 1986, it was added to the U.S. National Register of Historic Places. When Amtrak extended the Sunset Limited to Orlando in 1993 the nearest station with a stop was in Lake City. Today, only the former SAL line survives.

The Suwannee County Historical Museum is located in the depot. Exhibits include a telephone display, a 16th-century Timucaun Indian Village recreation and local history artifacts. The station is one block south of the Old Live Oak City Hall.