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The GenoType® MTBDRplus test is based on the DNA-Strip technology and permits the molecular genetic identification of the Mycobacterium tuberculosis complex and its resistance to rifampicin and/or isoniazid from cultivated samples or pulmonary smear-positive direct patient material.

Principle
The identification of rifampicin resistance is possible by the detection of the most significant mutations of the rpoB gene (coding for the β-subunit of the RNA polymerase). For testing of high level isoniazid resistance, the katG gene (coding for the catalase peroxidase) is examined and for testing of low level isoniazid resistance, the promoter region of the inhA gene (coding for the NADH enoyl ACP reductase) is examined.

Procedure
The whole procedure is divided into three steps:
 * DNA isolation
 * a multiplex amplification with biotinylated primers and
 * a reverse hybridization.

DNA could be isolated from