User:Abidur.rafi24/Evaluate an Article

Which article are you evaluating?
(G protein-coupled receptor)

Why you have chosen this article to evaluate?
G-Protein coupled receptors really interest me as numerous physiological processes and cellular responses are based upon GPCR interactions with various signaling molecules. Not to mention my current research focuses on GPCRs and I want to learn as much as possible. GPCRs are important because according to the article the ligands or signaling molecules that interact with the receptors are related to light-sensitivity, odors, pheromones, neurotransmitters, hormones and more, reflecting the idea that GPCRs are involved in numerous processes/actions within living organisms, particularly eukaryotes. As for my preliminary impression of the article, it appears to be well organized by initially providing an overview of what is known about GPCRs regarding its structure and role, also incorporating details regarding its history. Then moving onto more specifics related to the mechanism of function, signaling, the individual cAMP and PIP2 pathways and so on. The paper also appears to be greatly detailed providing a well-rounded overview of details and images reflecting the concepts being discussed. Additionally, the sources provided are vast and reputable, further aiding in the reliability of the information being reported within the article.

Evaluate the article
When reading the lead section, the article clearly describes the topic of discussion: GPCRs. It further goes onto briefly but accurately describing the articles major sections of discussion including ligands that interact with the GPCRs, the two distinct pathways involved within the signal transduction, the various signaling subunits involved within the process and more. However, receptor regulation is not clearly outlined within the lead section and would benefit the article if some note regarding it was made within the lead. In addition, the lead discusses applications of GPCRs as important drug targets and the applications within drug development, this is not discussed within later portions or the article in greater detail thus it would be beneficial for the readers to be provided additional details. Overall, the lead section provides enough detail to gain an overview of the article and its topics without overwhelming the reader with specific details but does lack noting an overview of GPCR regulation and includes details that are not later detailed regarding drug applications of GPCRs.

As for the content of the article, information is evenly distributed among the topics of discussion without placing too much emphasis on one compared to another. This is considering what is known and understood about each of the topics discussed, yes some sections provide more information compared to others, but this is to reflect what is known and unknown, despite this the length/details of sections do not take away from other portions of the discussion. As for the relevance of the content it is critical to understand the functions of GPCRs in addition to their structure and mechanism of function, without it GPCRs as a class of receptors will not be clearly understood, this would be a limitation to their study and the study of cellular processes/disease related. Additionally, the content related to signaling and regulation contributes to the overall understanding of GPCRs. As mentioned above, the applications of GPCRs as drug targets and drug development is something that needs to be further discussed within the article and is currently lacking. Moreover, most details within the article appear to be up-to-date utilizing sources within the 2000's and the oldest source dating back to 1987. However, there is discussion surrounding the signal transduction aspect discussed within this article that contrasts with current ideas of the transduction pathway involving a conformational selections, the author of the page should look more into this.

The tone of the article appears neutral as it doesn't focus on one point of view rather provides a wide outlook to the information and understanding regarding GPCRs. Additionally, there are no claims related to the understanding that appears heavily biased towards a particular point of view. However, the claims related to the importance of GPCRs as drug targets and their pharmaceutical applications appear underrepresented and should be provided more attention. Ultimately, the article is well written and does not take any bias towards discussing any side or detail.

When examining the sources used the links appear to work upon randomly selecting many and are readily available for viewing, and many of the ones listed supports the overall claims made within the article regarding understandings, in particular for GPCR structure/function relationships as well as mechanism of action. Additionally, the facts reported are all referenced appropriately with reliable secondary sources. And for the most part the sources are within the past 20 years, which may lend to the idea that there may be new studies on GPCRs that can contribute to this paper, despite this the sources listed do come from a diverse set of authors and publications lending to the neutral tone of the writing. Upon searching for additional sources that may contribute to this article "GPCR Signaling Regulation: The role of GRKs and Arrestins" by Vsevolod V. Gurevich and Eugenia V. Gurevich is one that may further or support the discussion regarding GPCR regulation. As for the limited discussion surrounding GPCRs as drug targets "G Protein-Coupled Receptors as Targets for Approved Drugs: How Many Targets and How Many Drugs?" by Krishna Sriram and Paul A. Insel, may provide useful details for the author of this page.

Again, the details and discussion are clearly organized, and the writing is concise, easy to read and understand. As for the images used, nothing that was used was distracting but contributed to the understanding of the topics and discussion points. While the images contribute to the readings understanding they are not as well-captioned as expected, some of the captions lack some details regarding the topic that an images is intended to reflect, for example the image captioned "G-protein-coupled receptor mechanism" the image focuses on that topic and it is understood, but readers may be confused as to what is happening in the figure, so a general overview may be helpful.

When viewing the talk-page discussions, as mentioned above there is a discussion surrounding the signal transduction mechanism where the article discusses a conformational change but current discussions may support the idea of a conformational selection rather than a change. Other discussions surround article formatting and placement of figures/images in the article, and some related to the references used. And further discussions relate to details presented within the article that may be confusing, or can be further developed for the readers understanding. The paper is rated as a level-5 vital article in Biology and is involved in Wiki projects for Molecular Biology / MCB, Neuroscience, and Biophysics. When reading the article, Wikipedia discusses the topic in a way that it does not command an extensive background knowledge from the reader, rather presents the information in a way that everyone can understand at a college level of education. Compared to class discussions, which may require a level of background knowledge especially knowing at least what a GPCR is and the different words used to describe processes and applications, and how we can draw comparisons between GPCRs and other cellular processes.

Overall the article is clearly written and concise and easy to understand and well-developed, those are its strengths. Despite this, the above mentioned comments should be taken into consideration to improve the quality of the content and understanding. To further the discussion surrounding GPCRs, I ask what impact might mutations on GPCRs have on cellular response, i.e those that involve neurotransmitters behaving as ligands? All in all, I enjoyed reading the article and look forward to any additions/edits to the page.