User:Acd88/Magnetofection

Lastly, an increase in transfection efficiency typically leads to an increase in cytotoxicity, which requires a balancing act, especially when adding transfection reagents like PEI.

Article body

 * Key gaps to fill
 * Disadvantages
 * OZ Biosciences trademark (expand (should be in Applications section)
 * Principle: (combine with lead section?)
 * Expand on “appropriate magnetic field” - is there a number? (see citation 3)
 * How much is a “significant vector dose”?
 * Applications
 * Expand / specify (subheadings for each?)
 * types of nucleic acids (DNA, siRNA, dsRNA, shRNA, mRNA, ODN)
 * non viral transfection systems (transfection reagents)
 * and viruses
 * Which cell lines?  / elaborate broad range
 * Hard-to-transfect cells (link to wiki or define)
 * Primary cells (link to wiki or define)
 * Several optimized and efficient magnetic nanoparticle formulations have been specifically developed for several types applications such as DNA, siRNA, and primary neuron transfection as well as viral applications
 * [citation needed]
 * List / describe formulations / applications
 * This seems to repeat the previous sentence?
 * Mechanism
 * Synthesis: Process of salt-induced colloidal aggregation and electrostatic interaction
 * How does magnetofection deliver nucleic acids to nucleus without cell division? Why primary and hard-to-transfect? Does it work for easy-to-transfect/dividing cells?
 * From magnet-assisted transfection The magnetic particles are accumulated in endosomes and/or vacuoles.
 * Biodistribution of Magnetic Nanoparticles
 * Proof - Not toxic
 * From magnet-assisted transfection Over time, the nanoparticles are degraded and the iron enters the normal iron metabolism. Influence of cellular functions by iron particles has not been reported yet. In most cases the increased iron concentration in culture media does not lead to cytotoxic effects.
 * What are Recommended doses and even higher doses
 * What are timings of other transfection methods? 10-15 minutes that is much faster than any other transfection method
 * Changes to organization
 * Mechanism
 * Split into 2 sections?: Production / synthesis and Mechanism
 * [citation] iron oxide fully biodegradable
 * Arbab https://doi.org/10.1148/radiol.2293021215
 * Potential subheadings (Synthesis)
 * Iron oxide /reagents
 * Salt-induced colloidal aggregation (page does not exist)
 * Electrostatic interaction
 * Potential subheadings (Mechanism)
 * Concentration onto target cells
 * Cellular uptake
 * Release into cytoplasm
 * Delivery to nucleus
 * Add proof of high transfection efficiency
 * Biodistribution of Magnetic Nanoparticles
 * Rename to Biocompatibility / Biodegradability
 * Biodistribution of Magnetic Nanoparticles
 * Rename to Biocompatibility / Biodegradability