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Pouilly-Fuissé is an appellation (AOC) for white wine in the Mâconnais subregion Burgundy in central France, located in the communes of Fuissé, Solutré-Pouilly, Vergisson and Chaintré. The AOC regulations only allow Chardonnay to be used. The allowed base yield is 50 hectoliter per hectare and the grapes must reach a maturity of at least 11.0 per cent potential alcohol.

West of Mâcon the land rises up to form Mont de Pouilly and other limestone hills, covered in the alkaline clay that best suits Chardonnay. The villages of Vergisson, Solutré-Pouilly, Fuissé and Chaintré shelter at their feet.

While Pouilly-Fuissé is the best-known part of Mâconnais, there are no Premier Cru vineyards within the AOC, as the local growers at the time the regulations were being drawn up never applied for this. The AOC was created on 11 September 1936. The area used to be known simply as "Pouilly", but when the AOC laws were introduced, it was split into three: Pouilly-Fuissé, Pouilly-Loché and Pouilly-Vinzelles.

In 2008, 757.20 ha of vineyard surface was in production within the Pouilly-Fuissé AOC, and 39,147 hectoliters of wine were produced, corresponding to 5.2 million bottles of wine. Despite the lack of official classification within the AOC, some climats within Pouilly-Fuissé have better reputations than others.

The wines of Pouilly-Fuissé should not be confused with the Sauvignon blanc-based wines of Pouilly-Fumé and the Chasselas-based Pouilly-sur-Loire, both from the area around Pouilly-sur-Loire in the Loire Valley.