User:AlexWiebe1/sandbox

Collection: Aquatic (water column)



The majority of recent environmental DNA studies focus on samples taken from the water column [1][2]. Recent studies have included tests with fishes, amphibians, aquatic mammals, and other organisms [2]. More data is needed to determine the ability of environmental DNA samples to estimate population sizes [3]. Currently, many studies use presence/absence data as the response variable from environmental DNA tests.

A volume of water (averaging ~2 L per sample) is filtered through a filter paper by use of a hand pump or peristaltic pump, and samples are stored for later laboratory analysis [4][5]. Samples filtered from water samples can be stored by freezing the samples or desiccating them in ethanol [6].



[1] Jerde, C.L., Mahon, A.R., Chadderton, W.L. and Lodge, D.M., 2011. “Sight‐unseen” detection of rare aquatic species using environmental DNA. Conservation Letters, 4(2), pp.150-157.

[2] Thomsen, P.F. and Willerslev, E., 2015. Environmental DNA–An emerging tool in conservation for monitoring past and present biodiversity. Biological Conservation, 183, pp.4-18.

[3] Evans, N.T., Olds, B.P., Renshaw, M.A., Turner, C.R., Li, Y., Jerde, C.L., Mahon, A.R., Pfrender, M.E., Lamberti, G.A. and Lodge, D.M., 2016. Quantification of mesocosm fish and amphibian species diversity via environmental DNA metabarcoding. Molecular Ecology Resources, 16(1), pp.29-41.

[4] Rees, H.C., Maddison, B.C., Middleditch, D.J., Patmore, J.R. and Gough, K.C., 2014. The detection of aquatic animal species using environmental DNA–a review of eDNA as a survey tool in ecology. Journal of Applied Ecology, 51(5), pp.1450-1459.

[5] Pilliod, D.S., Goldberg, C.S., Arkle, R.S. and Waits, L.P., 2013. Estimating occupancy and abundance of stream amphibians using environmental DNA from filtered water samples. Canadian Journal of Fisheries and Aquatic Sciences, 70(8), pp.1123-1130.

[6] Renshaw, M.A., Olds, B.P., Jerde, C.L., McVeigh, M.M. and Lodge, D.M., 2015. The room temperature preservation of filtered environmental DNA samples and assimilation into a phenol–chloroform–isoamyl alcohol DNA extraction. Molecular ecology resources, 15(1), pp.168-176.