User:Aly Ross/Cell suspension/Bpalanisamy Peer Review

General info

 * Whose work are you reviewing?

Alyssa Arnheim


 * Link to draft you're reviewing
 * https://en.wikipedia.org/wiki/User:Aly%20Ross/Cell_suspension?veaction=edit&preload=Template%3ADashboard.wikiedu.org_draft_template


 * Link to the current version of the article (if it exists)
 * Cell suspension

Evaluate the drafted changes
(Compose a detailed peer review here, considering each of the key aspects listed above if it is relevant. Consider the guiding questions, and check out the examples of what feedback looks like.)

Lead
This is awesome! I like how you defined what a cell suspension is. I think a picture showing the difference between cell suspensions vs. adherent cells would be nice, just because not all readers of Wikipedia are familiar with biology terms.

History
Amazing once again! I like how the sentences flowed with each other chronologically. Perhaps is there a way to add in dates or make a time line of events, so its easier for readers to understand without reading the entire paragraph.

Methods and Maintenance
In general I like how you make the classifications between different types of cultures


 * Isolating cells
 * Why are white blood cells able to be cultured in suspensions?
 * Suspensions
 * I think this part is great. How does the addition of surfactant descrease cell clumping? What kind of flasks do suspension cells have to be in? Can't I use a T75 flask but just place the flask in a shaker?
 * Spinner Flasks
 * I really liked how you explained what a spinner flask is. Perhaps a picture depicting your text would be a nice addition?
 * What are the advantages of a spinner flash compared with a normal flask?
 * Passaging
 * Trypan blue tells you which cells are alive/dead, but how can we know cell density?
 * Don't cell expand in an log-scale S shape instead of exponential?
 * This paragraph is solid as it descbires differences in passaging adherent vs. nonadherent cells

List of suspension cell lines
I really like how you included a table with all the cell lines.

General comments:


 * Overall this article is very well developed. Below are some suggestions
 * Including images that describe some of your writing would be helpful to the reader
 * I've only seen positive aspects of cell suspension, are there any negative aspects relative to adherent cells?