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Agilent 1100 is an analytical HPLC instrument manufactured by Agilent. Agilent specializes in manufacturing instrumentation for life sciences and chemical analysis, in addition to providing software and other services. Agilent 1100 is a modular system, which means that each module performs one particular function and is stacked on top of the other. A typical Agilent HPLC system has: pump module, auto sampler module, injector module, column module, and detector module.

Pump Module: The function of a pump is to force the mobile phase through the column at a specified flow rate (ml/min). A pump module can be a binary pump or a quaternary pump system. Both the binary and quaternary system are capable of generating an isocratic or a gradient elution.

Binary Pump: A binary pump system has two pumps and has the capability of pumping two different solvents to generate a gradient. Although, a binary pump system can be equipped with 4 different solvent bottles, however, at a particular instance a binary pump system can only pump two solvents to generate a gradient. The two solvents in a binary pump system are mixed using high pressure mixing. The mixing of the solvents happen after the solvents have been pumped using the pumps.

Quaternary pump: A quaternary pump has only one pump but it is equipped with the capability of mixing 4 different solvents to generate a gradient. Mixing of the solvents in quaternary system happens before the pump and it is categorized as low pressure missing. A degasser is a must in quaternary system because of low pressure mixing.