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Methods of generation
There are several methods for generating immortalised cell lines:


 * 1) Isolation from a naturally occurring cancer. This is the original method for generating an immortalised cell line. Major examples include human HeLa cells that were obtained from a cervical cancer, mouse Raw 264.7 cells that were subjected to mutagenesis and then selected for cells which are able to undergo division.
 * 2) Introduction of a viral gene that partially deregulates the cell cycle (e.g., the adenovirus type 5 E1 gene was used to immortalise the HEK 293 cell line; the Epstein-Barr virus can immortalize B lymphocytes by infection ).
 * 3) Artificial expression of key proteins required for immortality, for example telomerase which prevents degradation of chromosome ends during DNA replication in eukaryotes.
 * 4) Hybridoma technology, specifically used for generating immortalized antibody-producing B cell lines, where an antibody-producing B cell is fused with a myeloma (B cell cancer) cell.

Bibliography/Notes

 * Add citation to the methods section in Immortalised cell line. Not enough references are present for the relevant information presented.
 * Add citations to the point 1, 3, and 4 of the methods section with relevant articles.