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The 2025 NCAA Division I men's basketball tournament involved 68 teams playing in a single-elimination tournament to determine the National Collegiate Athletic Association (NCAA) Division I men's college basketball national champion for the 2024–25 season. The 86th annual edition of the tournament began on March 18, 2025, and concluded with the Illinois Fighting Illini becoming the lowest-seeded team to win the championship, defeating the Pittsburgh Panthers, 90-74 in the championship game on April 7, at the Alamodome in San Antonio, Texas.

Big South champion High Point made their NCAA tournament debut. Additionally, Louisiana Tech made their first appearance since 1991, Navy made their first appearance since 1998, and Arkansas State made their first appearance since 1999.

The Final Four consisted of UConn (third consecutive appearance), Illinois (first appearance since 2005), Pittsburgh (first appearance since 1941), and Xavier (their first Final Four appearance in program history).

Tournament procedure
A total of 68 teams entered the 2025 tournament. A total of 32 automatic bids were awarded to each program that win a conference tournament. The remaining 36 bids were issued "at-large", with selections extended by the NCAA Selection Committee. The Selection Committee seeded the entire field from 1 to 68.

Eight teams (the four-lowest seeded automatic qualifiers and the four lowest-seeded at large-teams) played in the First Four. The winners of these games advanced to the main tournament bracket.

2025 NCAA Tournament schedule and venues
The following are the sites selected to host each round of the 2025 tournament:.

First Four
 * March 18 and 19
 * University of Dayton Arena, Dayton, Ohio (Host: University of Dayton)

First and Second Rounds (Subregionals)
 * March 20 and 22
 * Amica Mutual Pavilion, Providence, Rhode Island (Host: Providence College)
 * Rupp Arena, Lexington, Kentucky (Host: University of Kentucky)
 * Intrust Bank Arena, Wichita, Kansas (Host: Wichita State University)
 * Ball Arena, Denver, Colorado (Host: Mountain West Conference)
 * March 21 and 23
 * Rocket Mortgage FieldHouse, Cleveland, Ohio (Host: Mid-America Conference)
 * Fiserv Forum, Milwaukee, Wisconsin (Host: Marquette University)
 * PNC Arena, Raleigh, North Carolina (Host: North Carolina State University)
 * Climate Pledge Arena, Seattle, Washington (Host: University of Washington)

Regional Semi-Finals and Finals (Sweet Sixteen and Elite Eight)
 * March 27 and 29
 * East Regional
 * Prudential Center, Newark, New Jersey (Host: Seton Hall University)
 * West Regional
 * Chase Center, San Francisco, California (Host: Pac-12 Conference)
 * March 28 and 30
 * South Regional
 * State Farm Arena, Atlanta, Georgia (Host: Georgia Institute of Technology)
 * Midwest Regional
 * Lucas Oil Stadium, Indianapolis, Indiana (Host: Horizon League, IUPUI)

National Semifinals and Championship (Final Four)
 * April 5 and 7
 * Alamodome, San Antonio, Texas (Host: University of Texas at San Antonio, University of the Incarnate Word)

San Antonio hosted the Final Four for the fifth time, having previously hosted in 2018.

Automatic qualifiers
Teams who won their conference championships automatically qualify.

Seeds
 The tournament seeds and regions were determined through the NCAA basketball tournament selection process and were published by the selection committee after the brackets were released on March 17. * See First Four Source: 

Tournament bracket
All times are listed in Eastern Daylight Time (UTC−4)

First Four – Dayton, Ohio
The First Four games involved eight teams: the four lowest-seeded automatic qualifiers and the four lowest-seeded at-large teams.

Midwest regional all-tournament team

 * PJ Hall (MOP) - Clemson
 * Mark Sears - Alabama
 * Grant Nelson - Alabama
 * Joseph Girard III - Clemson
 * Dillon Hunter - Clemson

West regional all-tournament team

 * Solomon Ball (MOP) - UConn
 * Hassan Diarra - UConn
 * Samson Johnson - UConn
 * Mohamed Diarra - NC State
 * Breon Pass - NC State

South regional all-tournament team

 * LJ Cryer (MOP) - Houston
 * J'Wan Roberts - Houston
 * Elliot Cadeau - North Carolina
 * Jalen Washington - North Carolina
 * RJ Davis - North Carolina

East regional all-tournament team

 * Ryan Kalkbrenner (MOP) - Creighton
 * Baylor Scheierman - Creighton
 * Cooper Flagg - Duke
 * Tyrese Proctor - Duke
 * Khaman Maluach - Duke

Tournament upsets
Per the NCAA, an upset occurs "when the losing team in an NCAA tournament game was seeded at least five seed lines better than the winning team."

The 2025 tournament saw a total of 13 upsets, with seven in the first round, five in the second round, and one in the Elite Eight.