User:Baylil00/Aquatic toxicology

History
While basic research in toxicology began in multiple countries in the 1800s, it was not until around the 1930s that the use of acute toxicity testing, especially on fish, was established. Due to the popularity of organochlorine pesticide DDT [l,l,l-trichloro-2,2-bis(p-chlorophenyl)ethane] and its linkage to causing fish death, the field of aquatic toxicology grew. At first, studies focused mainly on oysters and mussels, as they could not move away from the toxic environment. Over the next two decades, the effects of chemicals and wastes on non-human species became more of a public issue and the era of the pickle-jar bioassays began as efforts increased to standardize toxicity testing techniques.

In the United States, the passage of the Federal Water Pollution Control Act of 1947 marked the first comprehensive legislation for the control of water pollution and was followed by the Federal Water Pollution Control Act in 1956. In 1962, public and governmental interests were renewed, in large part due to the publication of Rachel Carson’s Silent Spring, and three years later the Water Quality Act of 1965 was passed, which directed states to develop water quality standards. Public awareness, as well as scientific and governmental concern, continued to grow throughout the 1970s and by the end of the decade research had expanded to include hazard evaluation and risk analysis. In the subsequent decades, aquatic toxicology has continued to expand and internationalize so that there is now a strong application of toxicity testing for environmental protection.

Currently, aquatic toxicology is continuing to evolve as risk assessment is becoming more practiced in the field. The field is gaining popularity as it has begun to link the effects of pollutants on marine animals to humans who eat fish and other marine life.

Aquatic Toxicity Tests
Aquatic toxicology tests (assays): toxicity tests are used to provide qualitative and quantitative data on adverse (deleterious) effects on aquatic organisms from a toxicant. Toxicity tests can be used to assess the potential for damage to an aquatic environment and provide a database that can be used to assess the risk associated within a situation for a specific toxicant. Aquatic toxicology tests can be performed in the field or in the laboratory. Field experiments generally refer to multiple species exposure, but single species can be caged for a set duration, and laboratory experiments generally refer to single species exposure. A dose–response relationship is most commonly used with a sigmoidal curve to quantify the toxic effects at a selected end-point or criteria for effect (i.e. death or other adverse effect to the organism). Concentration is on the x-axis and percent inhibition or response is on the y-axis.

Types of tests
Acute tests are short-term exposure tests (hours or days) and generally use lethality as an endpoint. In acute exposures, organisms come into contact with higher doses of the toxicant in a single event or in multiple events over a short period of time and usually produce immediate effects, depending on absorption time of the toxicant. These tests are generally conducted on organisms during a specific time period of the organism’s life cycle, and are considered partial life cycle tests. Acute tests are not valid if mortality in the control sample is greater than 10%. However, this control acceptability criterion is dependent upon the species and the duration of the test. Results are reported in EC50, or concentration that will affect fifty percent of the sample size.

Chronic tests are long-term tests (weeks, months years), relative to the test organism’s life span (>10% of life span), and generally use sub-lethal endpoints. In chronic exposures, organisms come into contact with low, continuous doses of a toxicant. Chronic exposures may induce effects to acute exposure, but can also result in effects that develop slowly. Chronic tests are generally considered full life cycle tests and cover an entire generation time or reproductive life cycle (“egg to egg”). Chronic tests are not considered valid if mortality in the control sample is greater than 20%. These results have generally been reported in NOECs (No observed effects level) and LOECs (Lowest observed effects level). However, NOECs and LOECs are becoming less common as endpoints are dependent on the concentration series chosen for the test. These reports are starting to become a topic of debate in the field because of the way it may alter the results of the tests. For example, if the concentration rate of the NOEC is 100, 50, 25, 11.25, 6.25 and the toxicology is reported at 2%, the NOEC would report the concentration as 6.25.

Sediment tests
At some point most chemicals originating from both anthropogenic and natural sources accumulate in sediment. For this reason, sediment toxicity can play a major role in the adverse biological effects seen in aquatic organisms, especially those inhabiting benthic habitats. A recommended approach for sediment testing is to apply the Sediment Quality Triad (SQT) which involves simultaneously examining sediment chemistry, toxicity, field alterations, bioaccumulation, and bioavailability assessments that can be used in a laboratory or in the field. Due to the expansion of SQTs, it is now more commonly referred to as "Sediment Assessment Framework." Collection, handling, and storage of sediment can have an effect on bioavailability and for this reason standard methods have been developed to suit this purpose