User:Biochemcsj

Dda
Dda (also known as DNA-dependent ATPase, Dda helicase, Dda DNA helicase) is the 49,897 Dalton 439 amino acid polypeptide coded by the Dda gene of the Enterobacteria phage T4.

Biochemistry
Dda hydrolyzes ATP in and only in the presence of substrate single stranded DNA. Dda binds its substrate (ssDNA) and processively translocates in a 5' to 3' direction in an ATP-dependent manner. In other words, Dda couples ATP hydrolysis and ssDNA translocation.

Coding Nucleotide Sequence  31,319 bases (32kb) from base 9410 to 10729

ATGACATTTGATGATTTGACCGAAGGCCAGAAAAATGCCTTTAACATTGTTATGAAGGCTATTAAAGAAA AGAAACATCATGTAACTATTAATGGACCTGCTGGTACCGGTAAGACTACTCTTACTAAGTTCATCATTGA AGCTTTAATATCTACGGGTGGAACTGGTATTATTTTAGCAGCTCCTACACATGCAGCTAAAAAGATTCTT TCAAAACTATCAGGGAAAGAAGCGAGTACTATTCATAGTATTCTTAAAATTAACCCAGTAACATATGAAG AAAATGTTCTTTTTGAACAAAAAGAAGTACCTGATTTAGCCAAATGCAGAGTATTAATCTGCGACGAAGT GTCAATGTATGATAGAAAGCTATTTAAAATTCTGCTTTCAACTATTCCACCTTGGTGTACTATAATTGGA ATAGGGGATAATAAGCAAATCAGACCTGTTGAACCAGGAGAAAATACTGCTTATATCAGTCCATTCTTTA CACATAAAGATTTTTATCAGTGTGAACTCACTGAAGTTAAACGCAGTAATGCTCCTATTATTGATGTAGC TACTGACGTTCGCAACGGTAAGTGGAATTATGATAAAGTTGTTGACGGGCATGGAGTACGTGGATTTACT GGTGATACCGCTTTACGCGATTTTATGGTAAATTATTTTTCAATCGTCAAATCACTAGATGATTTGTTTG AAAATCGCGTAATGGCATTTACGAATAAATCTGTTGACAAGTTAAATAGCATTATTCGTAAAAAGATTTT TGAAACTGATAAAGATTTTATTGTCGGTGAAATTATTGTAATGCAGGAACCATTATTTAAAACATATAAA ATTGATGGAAAGCCTGTGTCAGAAATTATTTTTAATAACGGACAATTAGTTCGTATTATAGAAGCAGAGT ATACATCAACGTTTGTTAAAGCCCGTGGTGTTCCTGGAGAATATCTAATTCGTCATTGGGATTTAACAGT AGAAACTTATGGCGATGATGAATATTATCGTGAAAAGATTAAAATAATTTCATCTGACGAAGAATTGTAT AAGTTTAACCTATTTTTAGCTAAAACAGCAGAAACTTATAAAAATTGGAACAAAGGCGGAAAAGCTCCGT GGAGTGATTTTTGGGATGCTAAATCACAGTTTAGTAAAGTGAAAGCACTTCCTGCATCAACATTCCATAA AGCGCAGGGTATGTCTGTAGACCGTGCTTTCATTTATACGCCTTGTATTCATTATGCAGATGTTGAATTA GCTCAACAACTTCTTTATGTTGGTGTCACCCGTGGTCGTTATGATGTGTTTTATGTATGA

Amino Acid Sequence

MTFDDLTEGQ KNAFNIVMKA IKEKKHHVTI NGPAGTGKTT LTKFIIEALI STGGTGIILA APTHAAKKIL SKLSGKEAST IHSILKINPV TYEENVLFEQ KEVPDLAKCR VLICDEVSMY DRKLFKILLS TIPPWCTIIG IGDNKQIRPV EPGENTAYIS PFFTHKDFYQ CELTEVKRSN APIIDVATDV RNGKWNYDKV VDGHGVRGFT GDTALRDFMV NYFSIVKSLD DLFENRVMAF TNKSVDKLNS IIRKKIFETD KDFIVGEIIV MQEPLFKTYK IDGKPVSEII FNNGQLVRII EAEYTSTFVK ARGVPGEYLI RHWDLTVETY GDDEYYREKI KIISSDEELY KFNLFLAKTA ETYKNWNKGG KAPWSDFWDA KSQFSKVKAL PASTFHKAQG MSVDRAFIYT PCIHYADVEL AQQLLYVGVT RGRYDVFYV

Molecular Biology
Dda is involved in the initiation of T4 DNA replication and DNA recombination.

Cellular Biology
Dda is toxic to cells at elevated levels.