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thumb|right|Shulin Zhuang (an artistic interpretation)


 * Shulin Zhuang
 * University of British Columbia
 * Mailing address:2036 Main Mall, Department of Chemistry, University of British Columbia, V6T 1Z1
 * Email:shulin.zhuang@gmail.com

As a postdoctoral fellow, now, I apply both computational methods and experimental methods in biophysics research field.

Objective
To find a position in industry or research institute where I can make contributions with my extensive experience in chemistry, molecular biology and computational biology field.

Highlights
1. Solid background in chemistry, molecular biology and computational biology, especially skilled in biophysics and molecular dynamics simulations.

2. Thorough knowledge in drug design & discovery, protein design.

3. Extensive experience in biophysics: AFM, CD, Fluorescence, Stop-Flow. 4. Strong skills in using computational soft: Amber, NAMD, Groamcs, Autodock, Dock, Sybyl, Modeller, Rosetta

Education

 * September 2007 ~ Present:       Postdoctoral fellow,  Department of Chemistry, University of British Columbia.

During this period, I perform both molecular biology experiment and computational biology work in biophysics research field to enhance protein mechanical properties.


 * March 2004 ~ March 2007:        Ph.D.,  Chemical Biology major, Department of Chemistry, Zhejiang University, China.

During this period, I use molecular modeling such as molecular dynamics, molecular docking and QSAR for drug design & discovery and the study of protein-ligand interactions.


 * September 2001 ~ February 2004: M.S.,   Organic Chemistry major, Department of Chemistry, Zhejiang University, China.

During this period, I have studied genomics and bioinformatics in Beijing Genomics Institute for four months and spent one year in James D.Watson Institue of Genome Sciences, Zhejiang University to complete the master thesis.


 * September 1999 ~ July 2001:     B.S.,   Chemistry Department, Qufu Normal University, China.


 * September 1997 ~ July 1999:     junior college student, Chemical education major, Linyi Normal University, China.

Research Experiences
September 2007 ~ Present:

I. Experimental project

1.Mapping unfolding pathway of TNfn3 by AFM Study mechanical properties and unfolding pathway of TNfn3 using bio-engineering technology.

2.Mechanism–based design of TNfn3 mutants with higher mechanical property Based on our own in silico unfolding study of TNfn3, build TNfn3 mutants with higher mechanical force with bio-engineering technology.

3.Mechanical study of Top7 with bihistidine engineering method Using bihistidine and disulfide bond engineering method to study the unfolding pathway and mechanical properties of Top7.

Methods:PCR, Electrophoresis, poly-protein building, protein expression and purification,AFM, CD, Fluorescence.

'''II. Computational project'''

1.Force-unfolding pathway study of GB1 and its two mutants:

To compare mechanical properties of GB1 and its two mutants as well as the elucidation of the unfolding pathway, conventional molecular dynamics simulations and steered molecular dynamics simulations(SMD) were performed. Results were analyzed using VMD and Python scripts.

2.Mechanical unfolding study of TNfn3

The unfolding pathway of TNfn3 was studied in explicit solvent by constant velocity and constant force SMD in atomic level.

3.Project in plan: Biomolecular design of proteins to enhance binding affinity

Explore the “hot spots” of the protein complex using Alanine-scanning and computationally design proteins to enhance the binding affinity.

Methods: constant velocity SMD, constant force SMD, conventional molecular dynamics simulations, principal component analysis, MM-PBSA, Alanine-scanning

March 2004 ~ March 2007:

1. “Unfolding of Macrophage Migration Inhibitory Factor(MIF) Domain with Steered Molecular Dynamics”. 2. “Molecular Dynamics Simulation and Free Energy Calculations of Macrophage Migration Inhibitory Factor(MIF)－inhibitor Complexes”

Methods: molecular dynamics simulations, molecular docking, steered molecular dynamics, locally enhanced sampling, MM-PBSA, QSAR

Doctoral thesis: “Molecular dynamics simulation of the interaction between macrophage migration inhibitory factor and small molecules”

January 2003 ~ February 2004:

“Screening for the molecular pathogenesis of autosomal dominant congenital stationary night blindness”, as a graduate student, hosted by Dr. Songnian hu at James D.Watson Institue of Genome Sciences of Zhejinag University, Hangzhou Genomics Institute

Methods: PCR, DNA sequencing

Master thesis: “Study of five point mutations and rhodopsin gene in a large Chinese family with autosomal dominant congenital stationary night blindness”

August 2002 ~ December 2002:

Attend the project of “The Genome Sequencing of Oryza sativa”, hosted by Dr. Songnian hu et al. at Beijing Genomics Institute.

Publications

 * 1) Qing Peng, Shulin Zhuang, Meijia Wang, Yi Cao, Yuanai Khor and Hongbin Li. Mechanical design of the third FnIII domain of tenascin-C. 2008, Submitted
 * 2) Yi Cao, Teri Yoo, Shulin Zhuang, Hongbin Li. Protein–protein interaction regulates proteins’ mechanical stability. J. MoL. Biol. 2008, 378, 1132-1141
 * 3) Shulin Zhuang, Jianwei Zou, Yongjun Jiang, Xiang Mao, Bing Zhang, Haichun Liu, Qingsen Yu. Some Insights into the Stereochemistry of Inhibition of Macrophage Migration Inhibitory Factor with 2-Fluoro-p-hydroxycinnamate and Its Analogues from Molecular Dynamics Simulations. J. Med.Chem. 2005，48, 7208-7214
 * 4) Xiaoling Liu, Shulin Zhuang, Songnian Hu, Feng Zhang, Bing Lin, Xu Li, D. Xu, Shi-Han Chen. A Dominant Form of Congenital Stationary Night Blindness (adCSNB) in A Llarge Chinese Family. Ann. Hum. Genet. 2005, 69, 315-321
 * 5) Jun Yu, Jun Wang, Wei Lin, Songgang Li …… Shulin Zhuang(the 61rst author)…… et al. The Genomes of Oryza sativa: A History of Duplications. PLoS Biol. 2005, 3(2), e38(0001-0016)
 * 6) Shulin Zhuang, Jianwei Zou, Chunlong Peng, Xiaoling Liu, Shihan Chen, Fangliang Huang, Songnian Hu,Qingsen Yu. Exclusion of the association of five known mutations with congenital stationary nyctalopia in a large Chinese family. Journal of Zhejiang University(medical sciences), 2005, 34(3), 255-259
 * 7) Shulin Zhuang, Qingsen Yu, Jianwei Zou, Yongjun Jiang, Yadong Chen, Huaxing Zhang, Haixiao Jin, Haichun Liu, Na Zhang. A Study of the Interaction of Cinnamate Analogues with Macrophage Migration Inhibitory Factor (MIF) and P1G Mutant from Molecular Dynamics Simulations. J. Mol. Struct.(THEOCHEM). 2006, 763，97-101
 * 8) Na Zhang, Yongjun Jiang, Jianwei Zou, Shulin Zhuang, Haixiao Jin, Qingsen Yu. Insights into unbinding mechanisms upon two mutations investigated by molecular dynamics study of GSK3β-axin complex: Role of packing hydrophobic residues. Proteins. 2007, 67(4), 941-949
 * 9) Bing Zhang, Vincent B. C. Tan, Kian Meng Lim, Tong Earn Tay, Shulin Zhuang. Study of the inhibition of cyclin-dependent kinases with roscovitine and indirubin-3’-oxime from molecular dynamics simulations. J. Mol. Model. 2006, 13(1), 79-89